收稿日期: 2009-12-14
网络出版日期: 2010-11-15
基金资助
国家高技术研究发展计划项目(2007AA021404, 2006AA10Z407)和转基因生物新品种培育科技重大专项(2009ZX08009-130B)资助
Upstream promoter sequences of Arabidopsis GH3 gene family
Received date: 2009-12-14
Online published: 2010-11-15
孙涛 , 柴团耀 , 张玉秀 . 拟南芥GH3基因家族启动子序列分析[J]. 中国科学院大学学报, 2010 , 27(6) : 847 -852 . DOI: 10.7523/j.issn.2095-6134.2010.6.017
GH3 genes belong to a primary auxin-response gene family. The 10 promoter sequences of Arabidopsis GH3 genes were analyzed using bioinformatics method. The results show that the transcription start site of these genes is generally 65~145bp away from the start codon, and the TATA boxes are located in the (-24)-(-40)bp. MDB and MatInspector analyses show that most upstream regions of these GH3 genes contain the cis-elements required for tissue and organ-specific expression responding to phytohormones and external environment, indicating that the expressions of GH3 genes are strictly controlled by multi-factors. Gene chip data show that AuxREs is very important for GH3 genes in response to IAA treatment,but it is not the unique cis-element for auxin response.
Key words： GH3 gene; promoter sequence; cis-element
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