收稿日期: 2010-04-09
修回日期: 2010-04-23
网络出版日期: 2011-01-20
基金资助
国家高技术研究发展计划(863)项目(2006AA10Z407, 2007AA021404)和中国科学院研究生院院长基金B类项目(085102FN00)资助
Cloning and expression analysis of PaAGAP gene from Phytolacca acinosa in response to various kinds of stress
Received date: 2010-04-09
Revised date: 2010-04-23
Online published: 2011-01-20
利用同源克隆和RACE方法在商陆( Phytolacca acinosa )中得到一个推测的小G蛋白ArfGTPase激活蛋白 ArfGAP (ArfGTPase activating protein) 的全长cDNA序列 PaAGAP . PaAGAP 序列编码332个氨基酸.蛋白含有保守的锌指结构域(CX2CX16CX2C类)和C2结构域.实时荧光定量PCR表明在寒、旱、盐和重金属的不同时间胁迫下,PaAGAP可以被诱导,表达量有不同程度的上调.由此推测 PaAGAP 可能是通过快速启动转录和翻译,使ARF失活,抑制植物生长素极性运输,来参与植物逆境反应的.
刘芳 , 吴亮其 , 王辉 , 张媛雅 , 柴团耀 . 商陆小G蛋白激活蛋白基因 PaAGAP 在逆境下表达研究[J]. 中国科学院大学学报, 2011 , 28(1) : 116 -124 . DOI: 10.7523/j.issn.2095-6134.2011.1.017
A full-length cDNA of an ArfGAP ( PaAGAP ) encoding 332 amino acids was cloned from Phytolacca acinosa by using methods of homology-based cloning and RACE. PaAGAP is predicted to contain a CX2CX16CX2C type zinc finger domain and a C2 domain. Real-time PCR analysis revealed that the expression of PaAGAP was up-regulated in response to low temperature, osmotic, salt, and heavy metal stresses, indicating a potential role of PaAGAP in the plant stress response that involves the inactivation of ARF and the inhibition of polar auxin transport.
Key words： Phytolacca acinosa; ArfGAP; zinc finger; stress; expression
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