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中国科学院大学学报 ›› 2011, Vol. 28 ›› Issue (1): 116-124.DOI: 10.7523/j.issn.2095-6134.2011.1.017

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商陆小G蛋白激活蛋白基因 PaAGAP 在逆境下表达研究

刘芳1, 吴亮其1, 王辉1, 张媛雅2, 柴团耀1   

  1. 1. 中国科学院研究生院,北京 100049;
    2. 中国矿业大学,北京100083
  • 收稿日期:2010-04-09 修回日期:2010-04-23 发布日期:2011-01-20
  • 基金资助:

    国家高技术研究发展计划(863)项目(2006AA10Z407, 2007AA021404)和中国科学院研究生院院长基金B类项目(085102FN00)资助 

Cloning and expression analysis of PaAGAP gene from Phytolacca acinosa in response to various kinds of stress

LIU Fang1, WU Liang-Qi1, WANG Hui1, ZHANG Yuan-Ya2, CHAI Tuan-Yao1   

  1. 1. College of Life Science, Graduate University, Chinese Academy of Sciences, Beijing 100049, China;
    2. China University of Mining & Technology, Beijing 100083, China
  • Received:2010-04-09 Revised:2010-04-23 Published:2011-01-20

摘要:

利用同源克隆和RACE方法在商陆( Phytolacca acinosa )中得到一个推测的小G蛋白ArfGTPase激活蛋白 ArfGAP (ArfGTPase activating protein) 的全长cDNA序列 PaAGAP . PaAGAP 序列编码332个氨基酸.蛋白含有保守的锌指结构域(CX2CX16CX2C类)和C2结构域.实时荧光定量PCR表明在寒、旱、盐和重金属的不同时间胁迫下,PaAGAP可以被诱导,表达量有不同程度的上调.由此推测 PaAGAP 可能是通过快速启动转录和翻译,使ARF失活,抑制植物生长素极性运输,来参与植物逆境反应的.

关键词: 商陆, ArfGAP, 锌指, 逆境, 表达

Abstract:

A full-length cDNA of an ArfGAP ( PaAGAP ) encoding 332 amino acids was cloned from Phytolacca acinosa by using methods of homology-based cloning and RACE. PaAGAP is predicted to contain a CX2CX16CX2C type zinc finger domain and a C2 domain. Real-time PCR analysis revealed that the expression of PaAGAP was up-regulated in response to low temperature, osmotic, salt, and heavy metal stresses, indicating a potential role of PaAGAP in the plant stress response that involves the inactivation of ARF and the inhibition of polar auxin transport.

Key words: Phytolacca acinosa, ArfGAP, zinc finger, stress, expression

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