收稿日期: 2010-05-17
修回日期: 2010-09-06
网络出版日期: 2011-05-15
基金资助
国家科技支撑计划(2009BAK61B01)、国家重点基础研究发展计划(2007CB935900)和中国科学院长春应用化学研究所电分析化学开放研究实验室基金(SKLEAC2010005)资助
Simultaneous determination of oxysophocarpine, sophoridine, and matrine by HPLC
Received date: 2010-05-17
Revised date: 2010-09-06
Online published: 2011-05-15
吴志娇 , 赵红 , 薛英 , 胡新明 , 毛一卿 , 谭焕然 . HPLC法同时测定氧化槐果碱、槐定碱和苦参碱[J]. 中国科学院大学学报, 2011 , 28(3) : 398 -401 . DOI: 10.7523/j.issn.2095-6134.2011.3.018
An HPLC method for simultaneous determination of oxysophocarpine, sophoridine, and matrine was proposed. VP-ODS column (150 mm × 4.6 mm, 5 μm) was used in the experiment. Column temperature was 40 ℃, mobile phase was 0.01 mol/L phosphate buffer solution (pH 8.5)-methanol (40 ∶60), UV detection wavelength was 215nm, and flow rate was 1.0 mL/min. The results indicated that the three kinds of alkaloid were separated effectively and showed good linearity within the selected concentration range.
Key words： oxysophocarpine; sophoridine; matrine; HPLC
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