收稿日期: 2002-01-07
修回日期: 2002-03-05
网络出版日期: 2002-07-18
基金资助
新疆维吾尔自治区自然科学基金项目(20001);乌鲁木齐重大科技攻关项目(Y0010 )资助
Study of the Mutation Breeding and Fermentation on Glutamic Acid Yielding Bacterium with Low Energy N+Ion Beam Implantation
Received date: 2002-01-07
Revised date: 2002-03-05
Online published: 2002-07-18
甄卫军 , 李茜 , 张石峰 , 曾宪贤 , 高林 , 李江 . 低能N+离子注入谷氨酸产生菌诱变选育及其发酵的研究[J]. 中国科学院大学学报, 2002 , 19(4) : 428 -432 . DOI: 10.7523/j.issn.2095-6134.2002.4.015
Low energy N+ion implantation technology is used in the mutat ion breeding and fermentation on g lutamic acid yielding start ing st rains of D110 and D110B.It is showed in this study that tw o high yielding glutamic acid st rains D5221 and B3263 are screened out and the average rocker bot tle fermentation GA(%)can achieve 8.4 %、7.5 %, they are respectively raised by 35.48 %、25 % compared to the original strains.There are clearly improved in the aspect on the fi rst class seed fermentation curve.And thei r log arithm phase has generally improved by 2 ～ 3 h.From the fermentation curve it is showed that thei r metabolic capacity are improved, the multiplicat ion cycle are shortened.So it w ill play a positive role in reducing the fermentation cycle.
Key words： ion implantation; glutamic acid; mutation breeding; fermentation
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