hCLP46是从MDS-AML的CD34＋细胞cDNA文库中筛选出的一个新的基因，为了进一步对该基因进行功能、性质研究，本实验利用RT-PCR技术对该基因进行了组织特异性表达检测，并将该基因构建到真核表达载体pcDNA3.1(+)中，转染U937细胞，发现过量表达该基因的U937细胞能够抑制TGF-对p15基因的上调。此外，利用生物信息学手段对该基因进行序列分析，得到其启动子区存在一个典型的“CpG island”。提示该基因有可能参与甲基化调节途径。通过本文的研究，为进一步研究该基因的作用机制打下基础。

hCLP46, a novel gene, has been screened out from the cDNA library of MDS-AML patient’s CD34+ stem cell. In order to further study the biological characters of the gene, RT-PCR was used to test the tissue specificity of hCLP46 gene expression. The results revealed hCLP46 was expressed in adult brain, not in fetal liver. After being sequenced, hCLP46 gene was cloned into XbaI~BamHI sites of pcDNA3.1. pcDNA3.1-hCLP46-myc was further transient transfected into U937 cells to detect the mechanisms of cell proliferation. It was shown that the expression of p15 inducted by TGF-βwas down regulated .By reference to gene sequence, the bio-informatics approach was used to elucidate the biology function of hCLP46, the analysis showed that a classical “CpG island” was found in the promoter of the gene which implied that the gene might be participated in methylation pathway.