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›› 2009, Vol. 26 ›› Issue (3): 319-322.DOI: 10.7523/j.issn.2095-6134.2009.3.005

• Research Articles • Previous Articles     Next Articles

Spectrophotometric determination of polyphenols in Quercus mongolicus Fisch leaves by means of Folin-ciocalteu reagent

ZHANG Guo-You1,2, TANG Ling1,2, CHEN Wei1, HE Xing-Yuan1, HUANG Wei1,2   

  1. 1. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;
    2. Graduate University, Chinese Academy of Sciences, Beijing 100049, China
  • Received:2008-04-01 Revised:2008-11-20 Online:2009-05-15

Abstract:

The total polyphenols(TP)content of Quercus mongolicus Fisch leaves extracts was analyzed by Folin-ciocalteu colorimetry,with gallic acid as standard.The method was improved and verified in the aspects of stability, linearity,precision and accuracy.The results showed that the total polyphenols content of Quercus mongolicus Fisch leaves extracts could be well calculated according to their colorimetric absorption at 760nm by applying Folin-ciocalteu reagent (1mol/L) 0.15mL and 10% volume fraction of Na2CO3 0.15mL at 25℃ for 80min. The (TP)content in Quercus mongolicus Fisch leaves determinated by the method was 6.39% and RSD was 1.90%.

Key words: Quercus mongolicus Fisch, polyphenol, Folin-ciocalteu, colorimetry

CLC Number: