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›› 2011, Vol. 28 ›› Issue (1): 57-64.DOI: 10.7523/j.issn.2095-6134.2011.1.009

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Effect of extracellular Ca2+ on osteoblast differentiation in MG-63 cells

ZHENG Jia-Qiang, YANG Jian-Hong   

  1. Graduate University, Chinese Academy of Sciences, Beijing 100049,China
  • Received:2010-04-29 Revised:2010-05-07 Online:2011-01-20

Abstract:

In the present study we have examined effects of extracellular Ca2+ on osteoblast differentiation in MG-63 cells.Theresults showed that5.0mmol/L Ca2+ up-regulated the expression of CaSR , OC、ALP、Runx 2、IGF-1 ,and BMP 2 . However,these indicators decreased after treatment with blocking agents. In addition,5.0 and 10.0mmol/L Ca2+ increased phospho-ERK expression, but did not affect ERK.Therefore 5.0mmol/L Ca2+ up-regulated CaSR and then promoted osteoblast differentiation through ERK pathway in MG-63 cells.

Key words: Ca2+, differentiation, osteoblast, CaSR, ERK

CLC Number: