Cloning and expression analysis of PaAGAP gene from Phytolacca acinosa in response to various kinds of stress

doi:10.7523/j.issn.2095-6134.2011.1.017

Abstract

Abstract:

A full-length cDNA of an ArfGAP ( PaAGAP ) encoding 332 amino acids was cloned from Phytolacca acinosa by using methods of homology-based cloning and RACE. PaAGAP is predicted to contain a CX₂CX₁₆CX₂C type zinc finger domain and a C2 domain. Real-time PCR analysis revealed that the expression of PaAGAP was up-regulated in response to low temperature, osmotic, salt, and heavy metal stresses, indicating a potential role of PaAGAP in the plant stress response that involves the inactivation of ARF and the inhibition of polar auxin transport.

Key words: Phytolacca acinosa, ArfGAP, zinc finger, stress, expression

CLC Number:

Q943.2

LIU Fang, WU Liang-Qi, WANG Hui, ZHANG Yuan-Ya, CHAI Tuan-Yao. Cloning and expression analysis of PaAGAP gene from Phytolacca acinosa in response to various kinds of stress[J]. , 2011, 28(1): 116-124.

References

[1] Cordulah. COP-coated vesicles in intracellular protein transport
[J].C Harter/FEBS Letters , 1995, 369: 89-92.

[2] Tomas K. Three ways to make a vesicle
[J]. Molecular Cell Biology , 2000, 12 (1): 187-198.

[3] Joel Moss, Martha Vaughan. Molecules in the ARF orbit
[J]. The Journal of Bioligical Chemistry , 1998, 273 (34): 21431-21434.

[4] Carolin W, Rainer B, Priska E,et al. Differential roles of ArfGAP1, ArfGAP2, and ArfGAP3 in COPI trafficking
[J]. J Cell Biol , 2008, 183 (4): 725-735.

[5] 吴静, 徐碧玉, 金志强. 小G蛋白的研究进展
[J].现代农业科技, 2007, 16: 195-197.

[6] Jensen R B,Lykke-Andersen K, Frandsen G I,et al. Promiscuous and specific phospholipid binding by domains in ZAC, a membrane-associated Arabidopsis protein with an ARF GAP zinc finger and a C2 domain
[J].Plant Molecular Biology , 2000, 44: 799- 814.

[7] Chong K, Zhuang X. ARF-GTPase as a molecular switch for polar auxin transport mediated by vesicle trafficking in root development
[J]. Plant Signaling & Behavior , 2007, 2 (2): 101-102.

[8] Zhuang X, Xu Y, Chong K,et al. OsAGAP , an ARF-GAP from rice, regulates root development mediated by auxin in Arabidopsis
[J].Plant Cell and Environment , 2005, 28: 147-156.

[9] Xue S G, Chen Y X, Lin Q, et al. Phytolacca acinosa Roxb . (Phytolaccaceae): A new manganese hyperaccumulator plant from Southern China
[J]. Acta Ecologica Sinica, 2003, 23 (5): 935-937 (in Chinese) . 薛生国, 陈英旭, 林琦,等. 中国首次发现的锰超积累植物商陆
[J]. 生态学报, 2003, 23 (5): 935-937.

[10] Tie B Q,Yuan M,Tang M Z. Phytolacca americana L.:A new manganese accumulator plant
[J]. Journal of Agro-Environment Science, 2005,24(2):340-343 (in Chinese). 铁柏清, 袁敏, 唐美珍. 美洲商陆( Phytolacca ameircaca L. )——一种新的Mn积累植物
[J]. 农业环境科学学报, 2005, 24 (2): 340-343.

[11] Nie F H, Wu C B, Wu S T. Characteristics of Phytolacca acinosa accumulating Cd
[J]. Journal of Zhejiang Forestry College, 2006,23(4):400- 405 (in Chinese). 聂发辉, 吴彩斌, 吴双桃. 商陆对镉的富集特征
[J]. 浙江林学院学报, 2006, 23 (4) : 400- 405.

[12] Bendtsen J D, Nielsenh, Vonh G, et al. Improved prediction of signal peptides: SignalP 3.0
[J]. J Mol Biol , 2004, 340 (4): 783-795.

[13] Nielsenh, Engelbrecht J, Brunak S,et al. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites
[J]. Protein Eng , 1997, 10 (1): 1-6.

[14] Hu R B,Fan C M,Fu Y F. Reference gene selection in plant real-time quantitative reverse transcription PCR (qRT-PCR)
[J]. Journal of Agricultural Science and Technology, 2009, 11(6): 30-36 (in Chinese). 胡瑞波, 范成明, 傅永福. 植物实时荧光定量PCR内参基因的选择
[J]. 中国农业科技导报, 2009, 11(6): 30-36.

[15] Memon A R. The role of ADP-ribosylation factor and SAR1 in vesicular trafficking in plants
[J]. Biochimica et Biophysica Acta (BBA)-Biomembranes , 2004, 1664 (1): 9-30.

[16] Reed R C, Brady S R, Muday G K. Inhibition of auxin movement from the shoot into the root inhibits lateral root development in Arabidopsis
[J]. Plant Physiol , 1998, 118: 1369-1378.

[17] Pasternak T, Rudas V, Potters G, et al. Morphogenic effects of abiotic stress: reorientation of growth in Arabidopsis thaliana seedlings
[J]. Environ Exp Bot , 2005, 53: 299-314.