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›› 2002, Vol. 19 ›› Issue (4): 428-432.DOI: 10.7523/j.issn.2095-6134.2002.4.015

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Study of the Mutation Breeding and Fermentation on Glutamic Acid Yielding Bacterium with Low Energy N+Ion Beam Implantation

ZHEN Wei Jun1, 2, LI Qian3, ZHANG Shi Feng3, ZENG Xian Xian3, GAO Lin1, LI Jiang4   

  1. 1. Xinjiang Chemical Institute of the Chinese Academy of Sciences, Urumqi 830011;
    2. The Gradudate School of the Chinese Academy of Sciences, Beijing 100039;
    3. Center of Ion Beam Bioengineering, Xinjiang University, Urumqi 830008;
    4. Xinjiang Shihezi Monosodium Glutamate Plant, Shihezi 832000
  • Received:2002-01-07 Revised:2002-03-05 Online:2002-07-18

Abstract:

Low energy N+ion implantation technology is used in the mutat ion breeding and fermentation on g lutamic acid yielding start ing st rains of D110 and D110B.It is showed in this study that tw o high yielding glutamic acid st rains D5221 and B3263 are screened out and the average rocker bot tle fermentation GA(%)can achieve 8.4 %、7.5 %, they are respectively raised by 35.48 %、25 % compared to the original strains.There are clearly improved in the aspect on the fi rst class seed fermentation curve.And thei r log arithm phase has generally improved by 2 ~ 3 h.From the fermentation curve it is showed that thei r metabolic capacity are improved, the multiplicat ion cycle are shortened.So it w ill play a positive role in reducing the fermentation cycle.

Key words: ion implantation, glutamic acid, mutation breeding, fermentation

CLC Number: