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›› 2016, Vol. 33 ›› Issue (3): 360-364.DOI: 10.7523/j.issn.2095-6134.2016.03.012

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Competitive enzyme-linked immunosorbent assay for rapid quantitative analysis of α-lactalbumin in bovine milk

SONG Hongxin, CHENG Ni, XUE Haiyan, DU Ruixuan   

  1. School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China
  • Received:2015-10-08 Revised:2016-03-01 Online:2016-05-15

Abstract:

A sensitive and specific competitive enzyme-linked immunosorbent assay (cELISA) for qualitative and quantitative analysis of α-lactalbumin (α-LA) is described. New Zealand white rabbits were immunised to obtain α-LA PcAb. By using sodium periodate oxidative method,the α-LA labeled by horseradish peroxidase (HRP) was prepared. Then cELISA was developed for α-LA quantitative analyses. The prepared PcAb has high specificity without obvious cross-reactivity and its titer is 1:80 920. The labeling rate of the enzyme-labeled antigen is 66.67%. The correlation coefficient of the established cELISA was 0.998 57, and the recoveries were estimated to be in the range from 86.00% to 117.50%. The variation coefficient is less than 5%. Compared with the national standard electrophoresis method,the established cELISA was useful analytical tool for rapid qualitative and quantitative analysis of α-LA in food samples.

Key words: quantitative analysis, proteins, cELISA, α-lactalbumin, immunization

CLC Number: