Study of syringe-free droplet microfluidic platforms enabling stable single-cell encapsulation

doi:10.7523/j.issn.2095-6134.2020.03.006

Abstract

Abstract: Negative pressures were used to generate droplets for single-cell encapsulations. Iodixanol was used to change the suspension solution densities in order to reduce the issue of cellular sedimentations. The effects of negative pressures and geometries of microfluidic T-channels on volumes and frequencies of generated droplets and the effects of iodixanol on the percentages of single-cell encapsulations were investigated and compared. This microfluidic platform may enable stable single-cell encapsulations, paving ways for single-cell analysis.

Key words: droplet microfluidics, single-cell encapsulation, negative pressure, iodixanol

CLC Number:

FAN Beiyuan, LIU Lixing, LI Xiufeng, CHEN Deyong, WANG Wenhui, WANG Junbo, CHEN Jian. Study of syringe-free droplet microfluidic platforms enabling stable single-cell encapsulation[J]. , 2020, 37(3): 336-344.

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