Cloning, expression and preparation of polyclonal antibody for human Dcp2 gene

doi:10.7523/j.issn.2095-6134.2007.3.015

›› 2007, Vol. 24 ›› Issue (3): 362-367.DOI: 10.7523/j.issn.2095-6134.2007.3.015

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Cloning, expression and preparation of polyclonal antibody for human Dcp2 gene

LIU Li-Xin, LIU Shu-Feng, Chang Nai-Bai, Liu Hui, Wang Wei

1: Graduate University of Chinese Academy of Sciences Beijing 100049，China；
2: Institute of Biophysics Chinese Academy of Sciences Beijing 100101， China；
3: Beijing Hospital Hematology Department Beijing 100073， China

Received:1900-01-01 Revised:1900-01-01 Online:2007-05-15

Abstract

Abstract: The degradation of eukaryotic mRNAs plays important roles in the modulation of gene expression, quality control of mRNA biogenesis. Dcp1/Dcp2 is a major component of mRNA decay factors. However, the more detail mechanisms of Dcp1/Dcp2 in mRNA degradation are unknown. In this study, our object is to amplify Dcp2 gene from human brain cDNA library, then to prepare rabbit-anti-human Dcp2 polyclonal antibodies. Our results showed that anti-Dcp2 rabbit polyclonal antibody was obtained. The antibody could detect the endogenous Dcp2 expression and its cell localization by both Western Blotting and immunohistochemistry, and Dcp2 protein also could be enriched by immunoprecipitation with this antibody. Therefore, this work provides a useful tool for further functional study of Dcp2 gene.

Key words: Dcp2, polyclonal antibody, Western blot, immunohistochemistry, immunoprecipitation

CLC Number:

Q786

LIU Li-Xin, LIU Shu-Feng, Chang Nai-Bai, Liu Hui, Wang Wei. Cloning, expression and preparation of polyclonal antibody for human Dcp2 gene[J]. , 2007, 24(3): 362-367.

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Cloning, expression and preparation of polyclonal antibody for human Dcp2 gene

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