A Study on Glutamate Oxaloacetate Transaminase Isozymes of Citrus Germplasm Resources

Zhong Guang-yan, Ye Yin-min, Chen Zhu-sheng, Wu Yun-lun

Received:1900-01-01 Revised:1900-01-01 Online:1993-03-10 Published:1993-03-10
Contact: Zhong Guang-yan

Abstract:

The glutamate oxaloacetate transaminase isozymes (GOT) extracted

from 125 biotypes of Citrus and its relatives, Fortunella, Poncirus and Microcitrus

were analyzed using polyacrylamide gel electrophoresis to investigate the taxonomic

relationships among citrus plants. Besides all the isozymes reported before, two new

bands were detected and designated their putative alleles B and C in GOT-l.

Among pummelo cultivars wide variations were found. Most of the mandarins

were identical, having SS for GOI-1 and MM for GOT-2 except for

Zou-Pi-Gan and Yao-Gan, which both had FS at GOT-1 and might be hy-

brids. This finding suggests that all of the mandarins may have originated from a

common ancestor. Sour orange biotypes showed a considerable variation in GOT

isozymes. Most of the sour oranges in China were assumed to be hybrids between

pummelo and mandarin based on GOT isozyme patterns, but two biotypes,

Xiao-Hong-Cheng and Zhu-lan, had FS at GOT-1 and MB at GOT-2, which

strongly suggests that they be hybrids of pummelo and Ichang papeda because B

allele of GOT-2 occurs only in Ichang papeda and its close relatives Yuzu and

Ichang lemon. From this study Yuzu is assumed to be derived from hybridization

of Ichan, papeda and mandarin.

Key words: Citrus, Poncirus, Fortunella, Microcitrus, GOT isozymes

Zhong Guang-yan, Ye Yin-min, Chen Zhu-sheng, Wu Yun-lun. A Study on Glutamate Oxaloacetate Transaminase Isozymes of Citrus Germplasm Resources[J]. .

[1]	Guo Tian-chi, Ye Yin-min. A New Species of Citrus (Rutaceae) from China [J]. , 1997, 35(4): 353-355.
[2]	Fang De-qiu, Zhang Wen-cai, Xiao Shun-Yuan. Studies on Taxonomy and Evolution of Citrus and Its Related Genera by Isozyme Analysis [J]. , 1993, 31(4): 329-352.
[3]	Zhong Guang-yan, Ye Yin-min. A Numerical Taxonomic Study of Citrus and Its Close Relatives [J]. , 1993, 31(3): 252-260.
[4]	Huang Yuan-Zheng, Chen Shu-Qun, He Chung-Ying, Chen Quan-You, Wu Yun-Lun. A Study on Chemical Components of Essential Oils from Citrus bergamia and Its Close Relatives and Its Taxonomy [J]. , 1992, 30(3): 239-244.
[5]	Zhong Guang-Yan, Ye Yin-Min. Study on Peroxidase Isozymes in Citrus and Fortunella [J]. , 1991, 29(5): 418-422.

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