Journal of University of Chinese Academy of Sciences >
Purification and Characterization of UGPase
Received date: 2003-07-10
Revised date: 2003-09-09
Online published: 2004-05-10
After isolation and purification of four steps, specific activity of UGPase from Apocynaceae cell is 204 9 U/mg. Compared with crude cell culture, specific activity is 97 times, and recovery yield is 21%. UGPase can be used in the synthesis of UDPG, and it has higher stability. The best available pH of UGPase is about 7 2, the best available temperature about 37℃. Mg2+ is necessary for UGPase to use. The high concentration of UTP and Glc 6 P can inhibit the activity of UGPase.
Key words： UGPase; purification; characterization; specific activity
QI Chao . Purification and Characterization of UGPase[J]. Journal of University of Chinese Academy of Sciences, 2004 , 21(3) : 345 -351 . DOI: 10.7523/j.issn.2095-6134.2004.3.010
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