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Spectrophotometric determination of polyphenols in Quercus mongolicus Fisch leaves by means of Folin-ciocalteu reagent
Received date: 2008-04-01
Revised date: 2008-11-20
Online published: 2009-05-15
The total polyphenols(TP)content of Quercus mongolicus Fisch leaves extracts was analyzed by Folin-ciocalteu colorimetry,with gallic acid as standard.The method was improved and verified in the aspects of stability, linearity,precision and accuracy.The results showed that the total polyphenols content of Quercus mongolicus Fisch leaves extracts could be well calculated according to their colorimetric absorption at 760nm by applying Folin-ciocalteu reagent (1mol/L) 0.15mL and 10% volume fraction of Na2CO3 0.15mL at 25℃ for 80min. The (TP)content in Quercus mongolicus Fisch leaves determinated by the method was 6.39% and RSD was 1.90%.
Key words： Quercus mongolicus Fisch; polyphenol; Folin-ciocalteu; colorimetry
ZHANG Guo-You , TANG Ling , CHEN Wei , HE Xing-Yuan , HUANG Wei . Spectrophotometric determination of polyphenols in Quercus mongolicus Fisch leaves by means of Folin-ciocalteu reagent[J]. Journal of University of Chinese Academy of Sciences, 2009 , 26(3) : 319 -322 . DOI: 10.7523/j.issn.2095-6134.2009.3.005
[1] Hou QF, Li DL, Li HT, et al. Study on microcolumn high performance liquid chromatography method for the determination of tea polyphenols. Food Science,2004,25(6):147~149(in Chinese) 胡秋芬,李德良,李海涛,等.微柱高效液相色谱法测定茶多酚的研究.食品科学,2004,25(6):147~149
[2] Cheng CL, Li JQ. Research advances in ecological significance and quantification of plant polyphenols. Chinese Journal of Applied Ecology,2006,17(2):2457~2460(in Chinese) 程春龙,李俊清.植物多酚的定量分析方法和生态作用研究进展. 应用生态学报,2006, 17 (12): 2457~2460
[3] Luo YF, Guo ZF, Zhu ZY, et al. Studies on ANN models of determination of tea polyphenol and amylose in tea by near-infrared spectroscopy. Spectroscopy and Spectral Analysis, 2005,25(8): 1230~1233(in Chinese) 罗一帆,郭振飞, 朱振宇,等. 近红外光谱测定茶叶中茶多酚和茶多糖的人工神经网络模型研究. 光谱学与光谱分析, 2005,25(8): 1230~1233
[4] Tang KW, Zhou CS, Zhong SA, et al. Study on adsorption of tea polyphenol and caffine with polyamide resin. Spectroscopy and Spectral Analysis, 2003,23(1):143~145(in Chinese) 唐课文,周春山, 钟世安,等. 聚酰胺树脂对茶多酚和咖啡因吸附选择性研究. 光谱学与光谱分析, 2003,23(1):143~145
[5] 冯建光,陈利琼.葡萄籽提取物中院花青素的测定.中国食品添加剂,2003(6):103~105
[6] Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidant substances and antioxidants by means of Folin-ciocalteu reagent. Methods in Enzymology,1999,299:152~178
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