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Screening proteins interacting with hCLP46 using tandem affinity purification
Received date: 2010-05-24
Revised date: 2010-05-27
Online published: 2011-03-15
hCLP46 (human CAP10-like protein46), a novel gene, has been screened out from the cDNA library of MDS-AML Patient’s CD34+ stem cell. For to further studying the biological characteristics of this gene tandem affinity purification (TAP) has been used to isolate proteins which specifically interact with hCLP46, and seven specific protein bands were detected. By using liquid chromatography-mass spectrometry and protein database searching, a series of endoplasmic reticulum chaperones were identified. Two major ER chaperone systems, the BiP/Grp94 and the calnexin (CNX)/ calreticulin (CRT) systems, are important in the maturation of hCLP46.
Key words： crosslinker; tandem affinity purification(TAP); MDS; hCLP46
XU Feng , MU Xin , WANG Wei , LIU Li-Xin . Screening proteins interacting with hCLP46 using tandem affinity purification[J]. Journal of University of Chinese Academy of Sciences, 2011 , 28(2) : 210 -216 . DOI: 10.7523/j.issn.2095-6134.2011.2.011
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