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Effects of Cd on activity and gene expression of antioxidant enzymes in hyperaccumulator Solanum nigrum L
Received date: 2012-03-16
Revised date: 2012-04-20
Online published: 2013-01-15
The 6-week-old seedlings of Solanum nigrum L. were treated with 30-100 μmol/L CdCl2 for 0-72 h. It was detected that the total activities of SOD, POD, CAT, and APX in leaf and root were obviously enhanced with increased Cd concentration and prolonged Cd treatment. Real-time quantitative PCR showed that the transcripts of FSD1, CSD2, POD2, and CAT2 in leaf or FSD1, CSD2, POD1, and APX3 in root were up-regulated and the mRNAs of POD1 and CAT1 in leaf or CSD1, MSD1, and CAT1 in root were down-regulated, while no changes in the transcripts of CSD 1, MSD1, APX1, and APX3 in leaf or POD2, CAT2, and APX1 in root were detected. The results indicate that the element Cd enhances or maintain the transcription level of antioxidant enzyme genes or regulates the post-transcriptional level, and it increases the enzyme activity.
Key words： Solanum nigrum L.; Cd; antioxidant enzyme; gene expression
ZHANG Yu-Xiu , JIN Ling , FENG Shan-Shan , LIU Jin-Guang , CHAI Tuan-Yao . Effects of Cd on activity and gene expression of antioxidant enzymes in hyperaccumulator Solanum nigrum L[J]. Journal of University of Chinese Academy of Sciences, 2013 , 30(1) : 11 -17 . DOI: 10.7523/j.issn.1002-1175.2013.01.003
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