Journal of University of Chinese Academy of Sciences >
Confirmation of weak protein-protein interactions by optimizing co-immunoprecipitation
Received date: 2012-02-23
Revised date: 2012-03-15
Online published: 2012-03-15
Co-immunoprecipitation is widely used to detect protein-protein interaction in physiological condition. The single-factor method was used to optimize some factors of co-immunoprecipitation, including component concentrations of cell-lysis buffer, antibody dosage, hCLP46 protein quantity, and cross-linking DSP. As a result, the weak interaction between hCLP46 (human CAP10-like protein 46) and ER chaperone calnexin is confirmed. The present work provides an important basis for further study of the protein-protein interaction.
FENG Xiao-Qin , XU Feng , WANG Wei , LIU Li-Xin . Confirmation of weak protein-protein interactions by optimizing co-immunoprecipitation[J]. Journal of University of Chinese Academy of Sciences, 2013 , 30(1) : 18 -23 . DOI: 10.7523/j.issn.1002-1175.2013.01.004
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