Journal of University of Chinese Academy of Sciences >
Interactions of β-amyloid peptide with metal ions studied by means of high-performance capillary electrophoresis
Received date: 2012-04-19
Revised date: 2012-05-02
Online published: 2012-05-02
Aggregation of the peptide amyloid-β (Aβ) into fibrils is considered to be closely related to the cause of Alzheimer disease (AD). Transition metals, such as Cu(Ⅱ), Zn(Ⅱ), and Fe(Ⅲ), are found in Aβ plaques and shown to affect Aβ aggregation. We investigated the interactions between those metal ions and the peptide amyloids (Aβ16 and Aβ28) by high-performance capillary electrophoresis coupled with UV detection (HPCE-UV). The experiments show that (i) the Zn2+, Cu2+, and Fe3+ ions interact with Aβ; (ii) Cu2+ rather than Zn2+ accelerates the aggregation of Aβ16 and the formation of oligomers. In addition, Zn2+ and Fe3+ accelerate the aggregation of Aβ28 with different acceleration rates.
YAO Fu-Jun , CUI Hong , LI Xiang-Jun . Interactions of β-amyloid peptide with metal ions studied by means of high-performance capillary electrophoresis[J]. Journal of University of Chinese Academy of Sciences, 2013 , 30(3) : 317 -321 . DOI: 10.7523/j.issn.1002-1175.2013.03.006
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