Cadmium (Cd) is one of the most toxic environmental pollutants for soils. The seedlings of S. nigrum were exposed to half-strength Hogland solution with 100 μmol/L CdCl2 for 1-4 days, and the activity of antioxidant enzyme in leaves and roots was investigated. The results show that the contents of MDA and H2O2 increase in leaves and roots and the increase of MDA in leaves is larger than that in roots while the increase of H2O2 in roots is larger than that in leaves. Also, the activities of SOD, CAT, APX, and POD in leaves and roots are enhanced with prolonged Cd treatment. It is suggested that the H2O2 accumulation induced by Cd stress leads to oxidative stress and the increase of the oxitioxidant enzyme activity may be one of the mechanisms of Cd detoxification in S. nigrum. H2O2 in leaves may be scavenged by both CAT and APX while the H2O2 in roots may be mainly scavenged by APX.
[1] Benzarti S, Hamidi H, Mohri S, et al. Response of antioxidative enzymes and apoplastic bypass transport in Thlaspi caerulescens and Raphanus satives to cadmium stress[J]. International Journal of Phytoremediation, 2010, 12(8): 733-744.
[2] Shi H, Wang Y, Tsang P E, et al. Alleviated affect of exogenous CaCl2 on the growth, antioxidative enzyme activities and cadmium absorption efficiency of Wedelia trilobata hairy roots under cadmium stress[J]. Chinese Journal of Biotechnology, 2012, 28(6): 747-762.
[3] Shi H, Tsang E P, Wang Y, et al. Effect of cadmium, alone or in combination with CaCl2,on the growth, antioxidative enzyme activity and cadmium absorption of Solanum nigrum L.var pauciflourum hairy roots[J]. Chinese Journal of Biotechnology, 2010, 26(2): 147-158.
[4] Sun W H, Wang W Q, Meng Q W. Functional mechanism and enzymatic and molecular characteristic of ascor-bate peroxidase in plants[J]. Plant Physiology Communications, 2005, 41(2): 143-147 (in Chinese). 孙卫红,王伟青,孟庆伟.植物抗坏血酸过氧化物酶的作用机制、酶学及分子特性[J]. 植物生理学通讯,2005, 41(2): 143-147.
[5] Podazza G, Arias M, Prado F E. Cadmium accumulation and strategies to avoid its toxicity in roots of the citrus rootstock Citrumelo[J]. Journal of Hazard Materials, 2012, 215-216: 83-89.
[6] Cho U H, Seo N H. Oxidative stress in Arabidopsis thaliana exposed to cadmium is due to hydrogen peroxide accumulation[J]. Plant Science, 2005, 168(1): 113-120.
[7] Liu Y T, Chen Z S, Hong C Y. Cadmium-induced physiological response and antioxidant enzyme changes in the novel cadmium accumulator, Tagetes patula [J]. Journal of Hazard Materials, 2011, 189(3): 724-31.
[8] Jin X, Yang X, Islam E, et al. Effects of cadmium on ultrastructure and antioxidative defense system in hyperaccumulator and non-hyperaccumulator[J]. Journal of Hazard Materials, 2008, 156 (1-3): 387-397.
[9] Sun R L, Zhou Q X, Sun F H, et al. Antioxidative defense and proline/phytochelatin accumulation in a newly discovered Cd-hyperaccumulator, Solanum nigrum L[J]. Environmental and Experimental Botany, 2007, 60(3): 468-476.
[10] Zhang X, Li C, Nan Z. Effects of cadmium stress on seed germination and seedling growth of Elymus dahuricus infected with the Neotyphodium endophyte[J]. Science China. Life Sciences, 2012, 55(9): 793-799.
[11] Martinez D D, Cordoba G F, Canalejo R A, et al. Cadmium-induced oxidative stress and the response of the antioxidative defense system in Spartina densiflora[J]. Physiologia Plantarum, 2010, 139(3): 289-302.
[12] Qiu R L, Zhao X, Tang Y T, et al. Antioxidative response to Cd in a newly discovered cadmium hyperaccumulator, Arabis paniculata F[J]. Chemosphere., 2008, 74(1): 6-12.
[13] Tang Y T, Guan L J, Qiu R L, et al. Antioxidative defense to cadmium in hyperaccumulator Picris divaricata V[J]. Acta Ecologica Sinica, 2010, 30(2): 324-332(in Chinese). 汤叶涛,关丽捷,仇荣亮, 等.镉对超富集植物滇苦菜抗氧化系统的影响[J].生态学报,2010,30(2): 324-332.
[14] Baker A J M, Whiting S N. In search of the Holy Grail-a further step in understanding metal hypera accumulation?[J]. New Phytologist, 2002, 155(1): 1-4.
[15] Wei S H, Zhou Q X, Wang X. Cadmium-hyperaccumulator Solanum nigrum L. and its accumulating characteristics[J]. Environmentai Science, 2005, 26(3): 167-171 (in Chinese).魏树和,周启星,王新. 超积累植物龙葵及其对镉的富集特征[J].环境科学,2005, 26(3): 167-171.
[16] Guo Z, Wang T, Ao Y S. Physiological responses of Solanum nigrum L. seedlings to cadmium stress[J]. Journal of Agro-Environment Science, 2009, 28(4): 755-760 (in Chinese). 郭智,王涛,奥岩松. 镉对龙葵幼苗生长和生理指标的影响[J].农业环境科学学报.2009, 28(4): 755-760.
[17] Zhang Y X, Huang Z B, Zhang H M, et al. Antioxidative response of phytolacca americana and Nicotiana tabacum to manganese stresses[J]. Environmentai Science, 2009, 30(12): 3 677-3 683 (in Chinese). 张玉秀, 黄智博, 张红梅, 等. 商陆和烟草对锰胁迫的抗氧化响应研究[J].环境科学,2009, 30(12):3 677-3 683.
[18] Pandey N, Singh G K. Studies on antioxidative enzymes induced by cadmium in pea plants (Pisum sativum)[J]. Journal of Environmental Biology, 2012, 33(2): 201-206.
[19] Daud M K, Sun Y, Dawood M, et al. Cadmium-induced functional and ultrastructural alterations in roots of two transgenic cotton cultivars[J]. Journal of Hazard Materials, 2009, 161(1): 463-473.
[20] Prasad M N V. Cadmium toxicity and tolerance in vascular plants[J]. Environmental Experiment Botany, 1995, 35(4): 525-545.
[21] Grant C A, Buckley W T, Bailey L D, et al. Cadmium accumulation in crops[J]. Canadian Journal of Plant Science, 1998, 78 (1): 1-17.