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Analysis of glutathione in cells by microchip electrophoresis coupled with electrochemical detection
Received date: 2013-04-07
Revised date: 2013-05-22
Online published: 2014-01-15
We established a new method for simultaneous determination of reduced and oxidized glutathione. The separation and detection conditions were optimized in terms of the buffer pH, buffer concentration, SDS concentration, separation potential, injection time, and detection potential. Under the optimized condition, GSH and GSSG were separated within 3 min, and the satisfactory linear ranges were 5~200 and 2~100 μmol/L for GSH and GSSG, respectively. The detection limits of GSH and GSSG were 4.87 and 1.98 μmol/L, respectively. Quantitative analyses of GSSG and GSH in cells were performed with satisfactory results.
YAO Lin , WU Liangqi , DING Yongsheng . Analysis of glutathione in cells by microchip electrophoresis coupled with electrochemical detection[J]. Journal of University of Chinese Academy of Sciences, 2014 , 31(1) : 38 -45 . DOI: 10.7523/j.issn.2095-6134.2014.01.007
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