Journal of University of Chinese Academy of Sciences >
Study on the Effect of Bacillus Megateriums in Two-Stage Fermentation of Vc
Received date: 2003-01-09
Revised date: 2003-03-31
Online published: 2004-01-10
Bacillus megaterium,as a companion strain in two-stage fermentation of vitamin C,could spur Gluconobacter ox ydans to grow and produce 2- ket o-L-gulonic acid( 2- KL G) the precursor of Vitamin C.In lab a mutated B. megaterium GB82 was screened from the original strain BP52 by low-energy ion implantation.When GAB,a mixed fermentation system which was com bined with G.oxydans and GB82,yielded 2- KLG from L-sorbose, the conversion rate could reach as high as 96%,about 10% higher than 2980,a mixed fermentation system which was combined with G.ox ydans and BP52. Studied and compared the effect of the active substances secreted by GB82 and BP52 on the ability of G.oxydans to produce 2-KL G. Hereby, suggested that the active substances be some proteins and the proteins be deactivated partly under extreme conditions.By means of Random Amplified Poly morphic DNA ( RAPD),also analyzed the genomic differences between BP52 and GB82 which were caused by low-energyion im plant ation.
Key words： Bacillus megateriums; two-stage fermentation of Vc; RAPD
Lv Shu-juan , Wang Jun , Yu Long , Xie Chuan-xiao , Gu Yun-hong , Yao Jian-ming , Yu Zeng-liang . Study on the Effect of Bacillus Megateriums in Two-Stage Fermentation of Vc[J]. Journal of University of Chinese Academy of Sciences, 2004 , 21(1) : 84 -89 . DOI: 10.7523/j.issn.2095-6134.2004.1.013
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