Journal of University of Chinese Academy of Sciences >
Studies on the Chemotaxis of a Bacterium Pseudomonas sp. Using Crude Oil as Carbon Source
Received date: 2004-06-08
Revised date: 2004-09-17
Online published: 2005-03-15
A bacterium Pseudomonas sp. using the crude oil as the carbon source was separated from the production water in an oilfield. Its chemotaxis migration ability was really medium, and its chemotaxis mechanism was particularly described in this paper. In visualized experiments, the ordered motion of microorganism to the oil water interface could be watched, and within the scope of the effect ive nutriment concentration grads, the chemotaxis mot ion velocity was not related with the distance from the interface at all and it was much influenced by the temperature. Meanwhile, in experiments using emulational micro-models, it could be observed that bacteria had become enriched at the orifice wall, oil and water three-phrase boundary, and the forming reason and the beneficial effect on MEOR were also discussed.
Key words： microorganism; chemotaxis; oil water interface; enrichment
JING Gui-Cheng , GUO Shang-Ping , YU Li . Studies on the Chemotaxis of a Bacterium Pseudomonas sp. Using Crude Oil as Carbon Source[J]. Journal of University of Chinese Academy of Sciences, 2005 , 22(2) : 187 -191 . DOI: 10.7523/j.issn.2095-6134.2005.2.010
[1] 'Parkinson JS. Protein phosphorylation in bacterial chemotaxis. Cell, 1988, 53( 1) : 1~ 2
[2] Bourret RB, Hess JF, Borkovich KA et al. Protein phosphorylat ion in chemotaxis and two-component regulatory syst ems of bact eria. J. Bi ol.Chem.1989, 264( 13) : 7085~ 7088
[3] Zhang WW. Zhang Z. The development of molecular mechanism of bacterial chemotaxis. Microbiol ogy, 1993, 20(3) : 175~ 179 ( in Chinese)
[4] Hess JF, Kenji O, Nachum K, et al. Phosphorylation of three proteins in the signal ing pathway of bacterial chemotaxis. 1988, Cell, 53( 1) : 79~ 87,
[5] Wolfe AJ, Conley MP, Tina JK, et al. Reconstitution of signaling in bact erial chemot axis. J. Bacteriol., 1987, 169(5) : 1878~ 1885
[6] SY'T 6242-1996 Al-l hydrocarbon gas-chromat ography on the contents of petroleum'paraffin'asphalt in crude oil. 2001
[7] Segal l JE, Ishihara A, Howard CB. Chemotact ic signal ing in f ilamentous cells of escherichia coli. J. Bacteriol., 1985, 161(1) : 51~ 59
[8] Stock AM, Mottonen JM, Schutt CE, et al. Three-dimensional structure of CheY, the response regulator of bacterial chemot axis. Nature( London), 1989, 337( 6209) : 745~ 749
[9] Lucassen-Reynders EH. Physical chemistry of surfact ant act ion ( Anionic surfactant s). Marcel Dekker inc., 1981. 102~ 113
[10] Li XW, Liu ZP. Microbial biodegradat ion of petroleum hydrocarbons. Acta Mi crobiologica Sinica. 2002, 42( 6) : 764~ 767 ( in Chinese withEnglish abstract)
[1] '张蔚文, 张'灼. 细菌化学趋向性机理的研究进展. 微生物学通报, 1993, 20( 3) : 175~ 179
[6] 'SY'T 6242-1996 原油中油质蜡质沥青质含量全烃气相色谱分析方法, 2001
[10] 李习武, 刘志培. 石油烃类的微生物降解. 微生物学报, 2002, 42( 6) : 764~ 767
/
| 〈 |
|
〉 |