Journal of University of Chinese Academy of Sciences >
Study of the Mutation Breeding and Fermentation on Glutamic Acid Yielding Bacterium with Low Energy N+Ion Beam Implantation
Received date: 2002-01-07
Revised date: 2002-03-05
Online published: 2002-07-18
Low energy N+ion implantation technology is used in the mutat ion breeding and fermentation on g lutamic acid yielding start ing st rains of D110 and D110B.It is showed in this study that tw o high yielding glutamic acid st rains D5221 and B3263 are screened out and the average rocker bot tle fermentation GA(%)can achieve 8.4 %、7.5 %, they are respectively raised by 35.48 %、25 % compared to the original strains.There are clearly improved in the aspect on the fi rst class seed fermentation curve.And thei r log arithm phase has generally improved by 2 ～ 3 h.From the fermentation curve it is showed that thei r metabolic capacity are improved, the multiplicat ion cycle are shortened.So it w ill play a positive role in reducing the fermentation cycle.
Key words： ion implantation; glutamic acid; mutation breeding; fermentation
ZHEN Wei Jun , LI Qian , ZHANG Shi Feng , ZENG Xian Xian , GAO Lin , LI Jiang . Study of the Mutation Breeding and Fermentation on Glutamic Acid Yielding Bacterium with Low Energy N+Ion Beam Implantation[J]. Journal of University of Chinese Academy of Sciences, 2002 , 19(4) : 428 -432 . DOI: 10.7523/j.issn.2095-6134.2002.4.015
[1] 王岁楼, 张一震, 李 志.谷氨酸高产菌TZ-310 的诱变选育及其发酵的研究.中国调味品, 1997,(9):16 ～ 18
[2] 宋道军, 余增亮.离子注入生物学.科学(中译本), 1996,(11):49 ～ 52
[3] 虞 龙, 许 安, 王 纪, 余增亮.低能离子注入在VC高产菌株选育中的应用.激光生物学报, 1998, 8(3):218 ～ 220
[4] 邱玉棠, 李 巍, 林小平.温度适应范围大的谷氨酸生产菌GMH-908 的选育、发酵及其应用效果.微生物学杂志, 1994, 15:17 ～ 22
[5] 张克旭, 高年发, 冯星如.关于谷氨酸高产菌WTH-1 菌的选育.食品与发酵工业, 1985,(3):15 ～ 21
[6] 方 序, 郭红樱, 陈 敏.高产谷氨酸菌株G1-1 菌的选育及生产试验.发酵科技通讯, 1989, 18(4):13 ～ 16
[7] 余增亮.离子束生物技术引论.合肥:安徽科学技术出版社, 1998.241 ～ 246
[8] 张克旭.氨基酸发酵工艺学.北京:中国轻工业出版社, 1997.79 ～ 87
/
| 〈 |
|
〉 |