Determination of methylated arginines in serum by a dispersive solid-phase extraction coupled with capillary electrophoresis-laser induced fluorescence detection

XIE Shijie; YANG Zecheng; ZHOU Zhou; LIN Yahui; SU Baoman; DING Yongsheng

doi:10.7523/j.issn.2095-6134.2020.04.005

Journal of University of Chinese Academy of Sciences >

2020 , Vol. 37 >Issue 4: 465 - 472

DOI: https://doi.org/10.7523/j.issn.2095-6134.2020.04.005

Research Articles

Determination of methylated arginines in serum by a dispersive solid-phase extraction coupled with capillary electrophoresis-laser induced fluorescence detection

XIE Shijie ,
YANG Zecheng ,
ZHOU Zhou ,
LIN Yahui ,
SU Baoman ,
DING Yongsheng

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1. College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China;
2. National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College&Testing Center, Fuwai Hospital, Beijing 100044, China

Received date: 2018-12-17

Revised date: 2019-05-08

Online published: 2020-07-15

Supported by

Supported by the National Nature Science Foundation of China (31571481,31771585)

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Abstract

We describe a dispersive solid-phase ion exchange extraction method combined with capillary electrophoresis-laser induced fluorescence detection for the determination of asymmetric dimethylarginine, symmetric dimethylarginine, and monomethylarginine in serum samples. The methylated arginine-extracted resin beads were added into a mixed solution of 4-chloro-7-nitrobenzofuran acetonitrile solution and alkaline sodium borate solution (pH=10. 5) for the derivatization in a 60℃ water bath. The three derivatives were subjected to baseline separation in a fused silica capillary under the conditions of a separation electrolyte of 80 mmol/L phosphate (pH=2. 0) and a separation voltage of 20 kV. On the basis of the FDA bioanalytical method validation guidance, the method validation was conducted and the results were satisfactory. This method was used to determine the three methylated arginines in the serum samples from local hospital.

Key words： methylated arginines; dispersive solid-phase extraction; 4-chloro-7-nitrobenzofurazan; capillary electrophoresis-laser induced fluorescence

Cite this article

XIE Shijie , YANG Zecheng , ZHOU Zhou , LIN Yahui , SU Baoman , DING Yongsheng . Determination of methylated arginines in serum by a dispersive solid-phase extraction coupled with capillary electrophoresis-laser induced fluorescence detection[J]. Journal of University of Chinese Academy of Sciences, 2020 , 37(4) : 465 -472 . DOI: 10.7523/j.issn.2095-6134.2020.04.005

References

[1] Bouras G, Deftereos S, Tousoulis D, et al. Asymmetric dimethylarginine (ADMA):a promising biomarker for cardiovascular disease?[J]. Current Topics in Medicinal Chemistry, 2013, 13:180-200.
[2] Kumarasamy C, Singh G, Raman P, et al. Effect of protein arginine methyltransferase-1 inhibition on hypoxia-induced vasoconstriction[J]. Medical Hypotheses, 2015, 85:740-743.
[3] Bajaj J S, Ahluwalia V, Wade J B, et al. Asymmetric dimethylarginine is strongly associated with cognitive dysfunction and brain MR spectroscopic abnormalities in cirrhosis[J]. Journal of Hepatology, 2013, 58:38-44.
[4] Inan B, Ates I, Ozkayar N, et al. Are increased oxidative stress and asymmetric dimethylarginine levels associated with masked hypertension?[J]. Clinical and Experimental Hypertension, 2016, 38:294-298.
[5] Wang Z, Tang W H, Cho L, et al. Targeted metabolomic evaluation of arginine methylation and cardiovascular risks[J]. Arteriosclerosis, Thrombosis, and Vascular Biology, 2009, 29:1383-1391.
[6] Wi?niewski J, Fleszar M G, Piechowicz J, et al. A novel mass spectrometry-based method for simultaneous determination of asymmetric and symmetric dimethylarginine, L-arginine and L-citrulline optimized for LC-MS-TOF and LC-MS/MS[J]. Biomedical Chromatography, 2017, 31:e3994.
[7] Kayacelebi A A, Knöfel A K, Beckmann B, et al. Measurement of unlabeled and stable isotope-labeled homoarginine, arginine and their metabolites in biological samples by GC-MS and GC-MS/MS[J]. Amino Acids, 2015, 47:2023-2034.
[8] Martens-Lobenhoffer J, Rodionov R N, Bode-Böger S M, et al. Determination of asymmetric Nα-acetyldimethylarginine in humans:a phase II metabolite of asymmetric dimethylarginine[J]. Analyical Biochemistry, 2014, 452:25-30.
[9] Kleparnik M, Tomandlova M, Glatz Z, et al. Determination of asymmetric and symmetric dimethylarginines in human plasma by HPLC with electrochemical detection[J]. Journal of Separation Science, 2013, 36:3696-3701.
[10] Davids M, Swieringa E, Palm F, et al. Simultaneous determination of asymmetric and symmetric dimethylarginine, L-monomethylarginine, L-arginine, andL-homoarginine in biological samples using stable isotope dilution liquid chromatography tandem mass spectrometry[J]. Journal of Chromatography B, 2012, 900:38-47.
[11] Shin S, Fung S M, Mohan S, et al. Simultaneous bioanalysis ofL-arginine,L-citrulline, and dimethylarginines by LC-MS/MS[J]. Journal of Chromatography B, 2011, 879:467-474.
[12] Gervasoni J, Bonelli F, Zuppi C, et al. Determination of asymmetric dimethyl arginine in human serum by liquid chromatography-tandem mass spectrometry:clinical application in hypertensive subjects[J]. Clinical Chemistry and Laboratory Medicine, 2011, 49:2109-2115.
[13] Brown C M, Becker J O, Wise P M, et al. Simultaneous determination of 6L-Arginine metabolites in human and mouse plasma by using hydrophilic-interaction chromatography and electrospray tandem massspectrometry[J]. Clinical Chemistry, 2011, 57:701-709.
[14] Gangi I M D, Chiandetti L, Gucciardi A, et al. Simultaneous quantitative determination of NG,NG-dimethyl-l-arginine or asymmetric dimethylarginine and related pathway's metabolites in biological fluids by ultrahigh-performance liquid chromatography/electrospray ionization-tandem mass spectrometry[J]. Analytica Chimica Acta, 2010, 677:140-148.
[15] Schwedhelm E, Maas R, Tan-Andresen J, et al. High-throughput liquid chromatographic-tandem mass spectrometric determination of arginine and dimethylated arginine derivatives in human and mouse plasma[J]. Journal of Chromatography B, 2007, 851:211-219.
[16] Yokoro M, Suzuki M, Yatani M, et al. Development of an enzyme-linked immunosorbent assay system for the determination of asymmetric dimethylarginine using a specific monoclonal antibody[J]. Bioscience Biotechnology and Biochemistry, 2012, 76:400-403.
[17] Zinellu A, Sotgia S, Zinellu E, et al. High-throughput CZE-UV determination of arginine and dimethylated arginines in human plasma[J]. Electrophoresis, 2007, 28:1942-1948.
[18] Linz T H, Snyder C M, Lunte S M. Optimization of the separation of NDA-derivatized methylarginines by capillary and microchip electrophoresis[J]. Journal of Laboratory Automation, 2012, 17:24-31.
[19] Zinellu A, Sotgia S, Usai M F, et al. Improved method for plasma ADMA, SDMA, and arginine quantification by field-amplified sample injection capillary electrophoresis UV detection[J]. Analytical and Bioanalytical Chemistry, 2011, 399:1815-1821.
[20] Teerlink T, Nijveldt R J, de Jong S, et al. Determination of arginine, asymmetric dimethylarginine, and symmetric dimethylarginine in human plasma and other biological samples by high-performance liquid chromatography[J]. Analytical Biochemistry, 2002, 303:131-137.
[21] Naz S, Garcia A, Rusak M, et al. Method development and validation for rat serum fingerprinting with CE-MS:application to ventilator-induced-lung-injury study[J]. Analytical and Bioanalytical Chemistry, 2013, 405:4849-4858.

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