Effect of chiral amino acids on DNA chain polymerization

BU Xinya; CHEN Lu; KAN Yuhe; LEI Huajun; HE Yujian; FENG Lutian; WU Li

doi:10.7523/j.issn.2095-6134.2021.03.003

Journal of University of Chinese Academy of Sciences >

2021 , Vol. 38 >Issue 3: 306 - 313

DOI: https://doi.org/10.7523/j.issn.2095-6134.2021.03.003

Review Article

Effect of chiral amino acids on DNA chain polymerization

BU Xinya ,
CHEN Lu ,
KAN Yuhe ,
LEI Huajun ,
HE Yujian ,
FENG Lutian ,
WU Li

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1. College of Applied Chemistry, Shenyang University of Chemical Technology, Shenyang 11014;
2. School of Chemical Sciences, University of Chinese Academy of Sciences, Beijing 100049, China;
3. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China

Received date: 2019-08-23

Revised date: 2019-09-17

Online published: 2021-05-17

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Abstract

The amino acids that make up natural proteins are L-conformation. Although D-conformation amino acids are not the basic structural units of proteins, many plants, microorganisms, and even human bodies have D-amino acids. DNA polymerase chain reaction occurs widely in organisms and in PCR. In this paper, the effect of chiral amino acids and their coordination with metal ions on DNA chain reaction catalyzed by Taq-DNA polymerase was investigated for the first time. It provides experimental basis for further study of the effects of chiral amino acids and metal ions on organisms. Denaturing polyacrylamide gel electrophoresis showed that acidic amino acids had inhibitory effect on DNA chain polymerization, and the polar uncharged amino acids. The inhibition of primer extension by L configuration was greater than that of D configuration amino acids, whereas the D structure of polar positively charged amino acids was opposite to that of L configuration. In the experiments of adding 9 metal ions such as Na⁺, K⁺, Ca²⁺, and chiral amino acids, the PCR was almost inhibited by aluminium and zirconium salts, followed by nickel, copper and calcium. When chiral amino acid metal complexes were added, the influence of chiral amino acid metal complexes on the results was partly changed.

Key words： chiral amino acids; metal ions; DNA chain polymerization; Taq-DNA polymerase

Cite this article

BU Xinya , CHEN Lu , KAN Yuhe , LEI Huajun , HE Yujian , FENG Lutian , WU Li . Effect of chiral amino acids on DNA chain polymerization[J]. Journal of University of Chinese Academy of Sciences, 2021 , 38(3) : 306 -313 . DOI: 10.7523/j.issn.2095-6134.2021.03.003

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