It is crucial for economic spatial structure to be industrial spatial division and specialization, and reasonable industrial spatial division takes advantage to use resource intensively and strengthen the regional competitiveness, and so on. Based on ways researching on industrial spatial division across the world, the article makes use of many ways, for example, industrial division exponential and specialization index and analyzes the whole manufacturing industrial division and division of important industry, taking the development zone along the river in Jiangsu as the sample. The article analyses the cause of industrial isomorphism based on H-O and industrial cluster theory. The conclusion is that the whole industrial division about development zone is clearly, and the information industry and metallurgy industry take on distinctly division. However, the partial industries are to some extent isomorphism, for instance, chemistry industry, mechanism industry and textile industry. The cause of isomorphism for chemistry industry and mechanism industry is the same for product elements. The cause of isomorphism for textile industry is that the area along Yangtze River in Jiangsu is traditional textile product zone. At the same time, it is still existing the problems on dispersing distribution of chemistry industry, smaller scope of mechanism industry and metallurgy industry, and so on. Finally, the article puts forward countermeasures to enhance spatial division of manufacturing industry in development zone along Yangtze River in Jiangsu.