Affinity chromatography (AC) technique，based on the affinity principle of ligand and receptor, as well as the specificity and reversibility of their combination, is a considerably efficient technique for high-throughput screening（HTS） of drugs. Since AC shows obviously the dynamical characters, associating and dissociating between ligands and receptors present in its column, it can present much more information than other related techniques. The information contained by its eluting curve reveals directly the interaction of ligand-target and its quantitative characterization, thereby presenting a convenience to drug discovery. Basing on the analysis of AC-based HTS, the present study establishes a series of qualitative and quantitative models. Because of their usability, these models are normally expected to play a positive role in the future on guiding the process of drug screening.