Objective We measured serum vascular endothelial growth factor and interleukin12 levels in acute leukemia samples and correlates these levels with disease characteristics and prognoses. The purpose of this research was to reveal the clinical relevance between VEGF and interleukin 12 of acute leukemia. Methods The serum VEGF and interleukin 12 levels were measured by quantitative Enzyme linked immunoassay (ELISA) which were applied to the groups of 10 untreated period, 10 complete remission period, 5 relapse period patients and 9 normal control. Results The concentrations of VEGF of untreated group(521.06± 163.85 pg/ml) were higher than complete remission(307.62±55.40 pg/ml) and normal group(262.01±141.66pg/ml). Differences existed between the concentrations of IL-12 in untreated group or relapse, complete remission and normal group. Negative correlation was found between the concentrations of VEGF and IL-12 in the normal group. The concentrations of VEGF and IL-12 were certain associated with the clinical condition of acute leukemia.