Function study of hCLP46 from human CD34+ stem/progenitor cells

ZHANG Tian; CHANG Nai-Bai; WANG  You-Xin; LIU  Hui; DU Jie; SONG  Man-Shu; LU Xiao-Mei; WANG Wei; LIU  Li-Xin

doi:10.7523/j.issn.2095-6134.2007.4.018

Journal of University of Chinese Academy of Sciences >

2007 , Vol. 24 >Issue 4: 520 - 524

DOI: https://doi.org/10.7523/j.issn.2095-6134.2007.4.018

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Function study of hCLP46 from human CD34+ stem/progenitor cells

ZHANG Tian ,
CHANG Nai-Bai ,
WANG You-Xin ,
LIU Hui ,
DU Jie ,
SONG Man-Shu ,
LU Xiao-Mei ,
WANG Wei ,
LIU Li-Xin

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1．Department of Biology, Graduate University of Chinese Academy of Science.
BeiJing 100049
2．Department of Hematology, BeiJing Hospital, Ministry of Health. BeiJing 100730
3．First hospital affiliated to XinJiang Medical University. Urumchi 830054

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2007-07-15

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Abstract

hCLP46, a novel gene, has been screened out from the cDNA library of MDS-AML patient’s CD34+ stem cell. In order to further study the biological characters of the gene, RT-PCR was used to test the tissue specificity of hCLP46 gene expression. The results revealed hCLP46 was expressed in adult brain, not in fetal liver. After being sequenced, hCLP46 gene was cloned into XbaI~BamHI sites of pcDNA3.1. pcDNA3.1-hCLP46-myc was further transient transfected into U937 cells to detect the mechanisms of cell proliferation. It was shown that the expression of p15 inducted by TGF-βwas down regulated .By reference to gene sequence, the bio-informatics approach was used to elucidate the biology function of hCLP46, the analysis showed that a classical “CpG island” was found in the promoter of the gene which implied that the gene might be participated in methylation pathway.

Key words： hCLP46; Myelodysplastic syndrome (MDS); CpG island; P15

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ZHANG Tian , CHANG Nai-Bai , WANG You-Xin , LIU Hui , DU Jie , SONG Man-Shu , LU Xiao-Mei , WANG Wei , LIU Li-Xin . Function study of hCLP46 from human CD34+ stem/progenitor cells[J]. Journal of University of Chinese Academy of Sciences, 2007 , 24(4) : 520 -524 . DOI: 10.7523/j.issn.2095-6134.2007.4.018

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