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Protective Ef fects of Glycerine on Mammal ian Cells in Ion Implantation

  • FENG HuiYun ,
  • YU Li Xiang ,
  • LIU Xing Hai ,
  • WU Li Jun
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  • Key Laboratory of Ion Beam Bioengineering of Chinese Academy of Sciences, Institute of Plasma Physics, Hefei 230031

Received date: 2002-01-07

  Revised date: 2002-03-22

  Online published: 2002-07-18

Abstract

The discovery of bio-ef fects induced by ion implantation has opened a novel branch in the field of radiation biology ———the low energy heavy ion biology.The experimental samples in the field have, however, been within the limits of plants and microbes because of the sample's vacuum-resistant ability.The mutation studying of mammalian cells with ion implantation hasn't been reported in the past years.The main reason for that is that it is difficult to keep mammalian cells alive in vacuum during ion implantation.In the present study, the tolerance of mammalian cells in vacuum was enhanced efficiently by pre-treatment with glycerin at low concent rat ion and proper treatment time, which provides a new way for the study on the biological effects of ion beam implantation in the mammalian cells.

Cite this article

FENG HuiYun , YU Li Xiang , LIU Xing Hai , WU Li Jun . Protective Ef fects of Glycerine on Mammal ian Cells in Ion Implantation[J]. Journal of University of Chinese Academy of Sciences, 2002 , 19(4) : 372 -375 . DOI: 10.7523/j.issn.2095-6134.2002.4.006

References

[1] Wang X Q, et al.Mass and Energy Deposition Effects of Implanted Ions on S olid Sodium Format e.Radiat Phys Chem, 2000, 59:67 ~ 70

[2] 杜严华等.不同能量氮离子束诱发DNA 链断裂的测量及量效曲线分析.自然科学进展, 2001, 11(6):577 ~ 581

[3] 丘冠英等.生物物理学.武汉:武汉大学出版社, 2000

[4] Yu Z L,et al.Ion Beam Application in Genet ic Modif icat ion.IEEE Transactions on Plasma Science, 2000, 28(1):128 ~ 132

[5] 吴丽芳等.用低能氩离子束介导将水稻几丁质酶基因导入小麦.科学通报, 2000, 45(21):2316 ~ 2321

[6] 姚建铭等.离子注入花生四烯酸产生菌诱变选育.生物工程学报, 2000, 16(4):478 ~ 481

[7] Waldren C A, et al.Measurement of mutagenesis in mammali an cells.Proc Natl Acad Sci USA, 1979, 76:1358 ~ 1362

[8] Waldren C A, et al.Measurement of low levels of x-ray mutagenesis in relation to human disease.Proc Nat l Acad Sci USA, 1986, 83:4839 ~4843

[9] Zhu L X, et al.Cellular and Molecular Analysis of Mu tagenesis Induced by Charged Particles of Defined Linear Energy Transf er.RadiationResearch, 1996, 145:251 ~ 259

[10] Yu Z L, et al.Dose Ef fect of the Tyrosine Sample Implant ed by Low Energy N + Ion Beam.Radiat Phys Chem, 1994, 43(4):349 ~ 351

[11] 李广斌等.低温生物学.长沙:湖南科学技术出版社, 1998

[12] 余增亮.离子束生物技术引论.合肥:安徽科学技术出版社, 1998

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