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镉对镉超累积植物龙葵抗氧化酶活性及基因表达的影响

张玉秀1, 金玲1, 冯珊珊2, 刘金光1, 柴团耀2   

  1. 1. 中国矿业大学(北京)化学与环境工程学院, 北京 100083;
    2. 中国科学院研究生院生命科学学院, 北京 100049
  • 收稿日期:2012-03-16 修回日期:2012-04-20 发布日期:2013-01-15
  • 通讯作者: 柴团耀
  • 基金资助:

    国家转基因生物新品种培育重大专项(2009ZX08009-130B)和中央高校基本科研业务费专项(2010YH05)资助

Effects of Cd on activity and gene expression of antioxidant enzymes in hyperaccumulator Solanum nigrum L

ZHANG Yu-Xiu1, JIN Ling1, FENG Shan-Shan2, LIU Jin-Guang1, CHAI Tuan-Yao2   

  1. 1. School of Chemical and Environmental Engineering, China University of Mining and Technology (Beijing), Beijng 100083, China;
    2 College of Life Science, Graduate University, Chinese Academy of Sciences, Beijing 100049, China
  • Received:2012-03-16 Revised:2012-04-20 Published:2013-01-15

摘要:

6周大的龙葵幼苗在30~100μmol/L CdCl2溶液中胁迫0~72h,叶和根SOD、POD、CAT和APX活性均随Cd浓度的提高和胁迫时间的延长而上升.实时定量PCR分析表明,100μmol/L CdCl2胁迫上调幼苗叶FSD1、CSD2、POD2CAT2与根FSD1、CSD2、POD1APX3的转录,下调叶POD1CAT1与根CSD1、MSD1CAT1的转录,而对叶CSD1、MSD1、APX1APX3与根POD2、CAT2APX1的表达水平无明显影响.这些结果表明Cd可增强或稳定多种抗氧化酶基因的转录水平或转录后水平的调节,提高酶活性.

关键词: 龙葵, 镉, 抗氧化酶, 基因表达

Abstract:

The 6-week-old seedlings of Solanum nigrum L. were treated with 30-100 μmol/L CdCl2 for 0-72 h. It was detected that the total activities of SOD, POD, CAT, and APX in leaf and root were obviously enhanced with increased Cd concentration and prolonged Cd treatment. Real-time quantitative PCR showed that the transcripts of FSD1, CSD2, POD2, and CAT2 in leaf or FSD1, CSD2, POD1, and APX3 in root were up-regulated and the mRNAs of POD1 and CAT1 in leaf or CSD1, MSD1, and CAT1 in root were down-regulated, while no changes in the transcripts of CSD 1, MSD1, APX1, and APX3 in leaf or POD2, CAT2, and APX1 in root were detected. The results indicate that the element Cd enhances or maintain the transcription level of antioxidant enzyme genes or regulates the post-transcriptional level, and it increases the enzyme activity.

Key words: Solanum nigrum L., Cd, antioxidant enzyme, gene expression

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