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中国科学院大学学报 ›› 2011, Vol. 28 ›› Issue (4): 551-555.DOI: 10.7523/j.issn.2095-6134.2011.4.017

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印度芥菜重金属ATP酶基因的分离与表达

张玉秀1, 张媛雅1, 柴团耀2   

  1. 1. 中国矿业大学化学与环境工程学院, 北京 100083;
    2. 中国科学院研究生院生命科学学院, 北京 100049
  • 收稿日期:2010-06-01 修回日期:2010-09-14 发布日期:2011-07-15
  • 基金资助:

    国家转基因生物新品种培育重大专项 (2009ZX08009-130B)和中央高校基本科研业务费专项资金(2010YH05)资助 

Isolation and expression of heavy metal ATPase in Brassica juncea L.

ZHANG Yu-Xiu1, ZHANG Yuan-Ya1, CHAI Tuan-Yao2   

  1. 1. School of Chemical and Environmental Engineering, China University of Mining and Technology, Beijing 100083, China;
    2. College of Life Science, Graduate University, Chinese Academy of Sciences, Beijing 100049, China
  • Received:2010-06-01 Revised:2010-09-14 Published:2011-07-15

摘要:

利用同源序列克隆技术在印度芥菜幼苗中分离出2个重金属ATP酶cDNA片段BjHMA 3BjHMA 4 ;实时荧光定量PCR表明BjHMA 3BjHMA4 在所有的组织器官中均有表达,其中在根中表达量最高,叶片中表达量最少;重金属Zn或Cd胁迫均能增强二者在叶片中的表达,表明BjHMA不仅参与植物的生长发育过程,而且在重金属稳态和耐性中具有重要作用.

关键词: 印度芥菜, 重金属ATP酶, 基因表达

Abstract:

Brassica juncea L. is a Zn/Cd tolerance plant, and the gene expression of heavy metal ATPase (HMA) has not been reported. In the present work, two cDNA fragments of BjHMA, BjHMA 3 and BjHMA 4 , were isolated from B. juncea seedling. Real-time quantitative PCR analysis revealed that both BjHMA 3 and BjHMA 4 were constitutively expressed in all tissue and preferentially in root. The mRNA profile of BjHMA 3 or BjHMA 4 was the lowest in leaf while the expressions were strongly enhanced by Zn or Cd stress, indicating that BjHMA played important roles in growth and in heavy metal homeostasis and tolerance in plant.

Key words: Brassica juncea L., heavy metal ATPase, gene expression

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