酵母细胞催化不对称合成D-扁桃酸甲酯的反应条件优化

doi:10.7523/j.issn.2095-6134.2005.2.011

中国科学院大学学报 ›› 2005, Vol. 22 ›› Issue (2): 192-196.DOI: 10.7523/j.issn.2095-6134.2005.2.011

酵母细胞催化不对称合成D-扁桃酸甲酯的反应条件优化

盛军^1,2, 郭养浩², 石贤爱¹, 肖美添², 孟春¹

1. 福州大学生物科学与工程学院, 福州 350002;
2. 福州大学药物生物技术与工程研究所, 福州 350002

收稿日期:2004-06-09 修回日期:2004-08-16 发布日期:2005-03-15
通讯作者: 孟春,E-mail:mengchun@fzu.edu.cn
基金资助:
福建省科技厅项目(QX20024)资助

Optimization of Reaction Conditions in the Asymmetric Synthesis of D-Mandelic Acid Methyl Ester Catalyzed by Saccharomyces Cereviriae

SHENG Jun^1,2, GUO Yang-Hao², SHI Xian-Ai¹, XIAO Mei-Tian², MENG Chun¹

1. College of Biological Science and Technology, Fuzhou University, Fuzhou 350002, China;
2. Institute of Pharmaceutical Biotechnology and Engineering, Fuzhou University, Fuzhou 350002, China

Received:2004-06-09 Revised:2004-08-16 Published:2005-03-15

摘要/Abstract

摘要：

采用酿酒酵母催化苯乙酮酸甲酯不对称合成D扁桃酸甲酯,其过程的最佳转化pH值为70左右.酵母生长代谢环境中的温度和氧水平对底物转化均有较大影响.采用固定化细胞填充床反应器转化苯乙酮酸甲酯,每克酵母湿菌体进料负荷为0.2mmold,成功运行25d,转化率保持在80%以上,D扁桃酸甲酯ee值达95%以上.

关键词: 酿酒酵母, 不对称合成, D扁桃酸甲酯, 氧水平

Abstract:

In the process of asymmetric synthesis of D-mandelic acid methyl ester catalyzed by Saccharomyces cereviriae, the most favorite pH for conversion is 7.0.Both the environmental temperature and oxygen concentration all have an important effect on conversion of substrate.Using packed bed reactor filled with immobilized cells to produce D-mandelic acidmethyl ester,25 days later the conversion efficiency can be still sustained in 80 %and the ee value is more than 95 %when the charge was 0.2 mmol (d·g)wet cell.

Key words: Saccharomyces cereviriae, asymmetric synthesis, D-mandelic acid methyl ester, oxygen level

中图分类号:

TQ9201

盛军, 郭养浩, 石贤爱, 肖美添, 孟春. 酵母细胞催化不对称合成D-扁桃酸甲酯的反应条件优化[J]. 中国科学院大学学报, 2005, 22(2): 192-196.

SHENG Jun, GUO Yang-Hao, SHI Xian-Ai, XIAO Mei-Tian, MENG Chun . Optimization of Reaction Conditions in the Asymmetric Synthesis of D-Mandelic Acid Methyl Ester Catalyzed by Saccharomyces Cereviriae[J]. , 2005, 22(2): 192-196.

参考文献

[1] Lin GQ, Chen YQ,CHEN XZ.Chiral synthesis —Asymmetric synthesis and application.Bei jing :Science Press, 2000.2～ 17(in Chinese)

[2] Fukuit, Kawamotot, Tanaka A.Tetrahedron :enzymati c preparation of optical ly active silylmethanol derivatives having a stereogeni c silicon atom byhydrolase-catalyzed enantioselective esterif ication.Asymmetry, 1994, 5(1):73 ～ 82

[3] Pan BF, Shi YP, Li ZY.Asymmetry bio-produce chiral medi cine.Industry Microbe, 1998, 28(4):42 ～ 45 (in Chinese with English abstract)

[4] Huang NP, Zhong P.Stero and chemoselect ive reduction of ketones and aldenhydes using Baker's Yeast.Jiangxi Science, 2002, 20 :59 ～ 62(inChinese with English abstract)

[5] Ji YB, Chen YY, Lin BC.Chiral separation of basic drugs in cyclodextrin modified capillry zone elect rophorsis.Journal of China MedicineUniversity, 1996, 27(4):230 ～ 234(in Chinese with English abst ract)

[6] Ruan ZQ, Kang JW, Ou QY.A study on the binding of cyclodextrins with terbutaline enantiomers by chi ral capilary zoneelectrophoresis,Chromatogram, 1998,16(6):481 ～ 484(in Chinese with English abstract)

[7] Zhu WZ, Xu JH, Yu JT, Asymmetry bioreduction of ethylAcetoacetate catalyzed by Baker's Yeast.Journal of East ChinaUniversity of Science andTechnology, 2000, 26:154 ～ 156(in Chinese with English abstract)

[8] Liu X, Sun PD, Li M.Asymmetri c reduct ion of acetophenone with Baker's Yeast.Molecule Catalyze, 2002, 16(2):107 ～ 110(in Chinese)

[9] Yang Z, Yu LY, Song J, et al.Study on the primary screening model for the aldose reductase inhibitors.Chinese Journal of Antibiotics, 2002, 27(4):193 ～ 196(in Chinese with Engli sh abstract)

[10] Willner, D Mandler.Enzyme-catalysed biotransformations through photochemical regeneration of ni cotinamide cofactors.Enzyme Microb.Technol., 1989, 11(8):467 ～ 483

[11] Chen SY.Yeast Biochemistry.Jinan :Shandong Science and Technology Press, 1990.107 ～ 115(in Chinese)

[1] 林国强, 陈耀全, 陈新滋.手性合成———不对称合成及其应用.北京:科学出版社, 2000.2 ～ 17

[3] 潘冰峰, 施邑屏, 李祖义.不对称生物还原制备手性药物.工业微生物, 1998,28(4):42～ 45

[4] 黄南平, 钟平.面包酵母的立体选择性还原作用.江西科学, 2002, 20 :59 ～ 62

[5] 季一兵, 陈玉英, 林炳承.若干手性药物的环糊精系统毛细管区带电泳拆分.中国药科大学学报, 1996,27(4):230 ～ 234

[6] 阮宗琴, 康经武, 欧庆瑜.毛细管电泳环糊精添加剂拆分特布他林对映体的立体选择性研究.色谱, 1998, 16(6):481 ～ 484

[7] 朱文洲, 许建和, 俞俊棠.面包酵母催化乙酰乙酸乙酯的不对称还原反应.华东理工大学学报, 2000, 26:154 ～ 156

[8] 刘湘, 孙培冬, 李明.面包酵母用于苯乙酮的不对称还原研究.分子催化, 2002, 16(2):107 ～ 110

[9] 杨, 余利岩, 宋京, 等.醛糖还原酶抑制剂筛选模型的建立和初步应用.中国抗生素杂志, 2002, 27(4):193 ～ 196

[11] 陈思.酵母生物化学.济南:山东科技出版社, 1990.107 ～ 115

酵母细胞催化不对称合成D-扁桃酸甲酯的反应条件优化

Optimization of Reaction Conditions in the Asymmetric Synthesis of D-Mandelic Acid Methyl Ester Catalyzed by Saccharomyces Cereviriae

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