黑曲霉Aspergillus nigerS菌株所产β-葡糖苷酶 的纯化和酶学性质

doi:10.7523/j.issn.2095-6134.2009.2.018

摘要/Abstract

摘要：

利用黑曲霉Aspergillus niger S菌株, 通过液体发酵的方法, 得到了一种可同时高效水解大豆异黄酮糖苷成大豆异黄酮,水解白藜芦醇苷成白藜芦醇的糖基水解酶.该酶经硫酸铵分级沉淀、离子交换层析及分子排阻色谱纯化得单一条带, 经SDS聚丙烯酰胺凝胶电泳测得该酶的表观分子量约为165ku 1u=1.660540×10^-27kg。 ,比通常所报道的传统β-葡糖苷酶都大,该酶的最适反应温度为50℃; 最适pH值为5~10.

关键词: 大豆异黄酮, 白藜芦醇, 葡糖苷酶, 纯化, 性质

Abstract:

A high activity glucosidase, which can hydrolyze glycosides, was isolated from Aspergillus niger S strain. The enzyme was purified to one spot after ammonium sulfate precipitation, ion exchange & size exclusion chromatography and its molecular weight was about 165ku, which was quite differ from other reported glucosidase. The optimum temperature of this enzyme was 50℃ & the optimum pH was pH 5~10. Based on the HPLC result, this enzyme can transform glycone to aglycone of isoflavones & resveratrol with high specific activity.

Key words: soybean isoflavones, resveratrol, glucosidase, purification, characterization

中图分类号:

王云, 曾沃坦. 黑曲霉Aspergillus nigerS菌株所产β-葡糖苷酶的纯化和酶学性质[J]. 中国科学院大学学报, 2009, 26(2): 274-279.

WANG Yun, ZENG Wo-Tan. Purification and characterization of β-glucosidase from Aspergillus niger[J]. , 2009, 26(2): 274-279.

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