商陆寡肽转运蛋白基因PaOPT的 cDNA全序列克隆及表达分析

doi:10.7523/j.issn.2095-6134.2012.3.006

中国科学院大学学报 ›› 2012, Vol. ›› Issue (3): 316-323.DOI: 10.7523/j.issn.2095-6134.2012.3.006

商陆寡肽转运蛋白基因PaOPT的 cDNA全序列克隆及表达分析

金力¹, 郑宏春^1,2, 柴团耀¹

1. 中国科学院研究生院生命科学学院, 北京 100049;
2. 陕西教育学院生物科学与技术系, 西安 710100

收稿日期:2011-04-28 修回日期:2011-05-10 发布日期:2012-05-15
通讯作者: 郑宏春
基金资助:
国家高技术研究发展计划(863计划)(2006AA10Z407)和转基因生物新品种培育科技重大专项(2009ZX08009-130B) 资助

Cloning and expression of PaOPT in Phytolacca americana

JIN Li¹, ZHENG Hong-Chun^1,2, CHAI Tuan-Yao¹

1. College of Life Science, Graduate University, Chinese Academy of Sciences, Beijing 100049, China;
2. Department of Life Science and Technology, Shaanxi Institute of Education, Xi'an 710100, China

Received:2011-04-28 Revised:2011-05-10 Published:2012-05-15

摘要/Abstract

摘要： 从垂序商陆(Phytolacca americana)的重金属镉胁迫下商陆幼苗叶片的正反向抑制性消减杂交文库(SSH文库)中,获得商陆寡肽转运蛋白基因的EST序列. 利用RACE的方法克隆到该基因的全序列cDNA(PaOPT,GenBank注册HQ647015). PaOPT基因在进化中的地位分析表明:它可能属于OPT 3 基因家族. 利用实时荧光定量PCR的方法,分析PaOPT的组织表达特异性以及商陆种子成熟及萌发过程的表达模式. 结果表明,该基因是一个在种子中特异性表达的基因,预测它对植物的生长发育有着重要的作用.

关键词: 商陆, 寡肽转运蛋白, 表达模式

Abstract: An oligopeptide transporter gene was isolated from Cd-treated leaf tissue of Phytolacca americana by SSH method, designated by PaOPT (GenBank accession No. HQ647015). The full length of this gene has been cloned by race-PCR technique. Moreover, the evolution of PaOPT in OPT family and the expression profiles during seed germination period was investigated. Our study indicates that PaOPT belongs to the OPT3 family. The results of real-time quantitative PCR show that PaOPT may be a seed-specific expression gene. Further investigation of the function and localization of PaOPT will be favorable for understanding of plant growth and development.

Key words: Phytolacca Americana, PaOPT, expression

中图分类号:

Q945.3

金力, 郑宏春, 柴团耀. 商陆寡肽转运蛋白基因PaOPT的 cDNA全序列克隆及表达分析[J]. 中国科学院大学学报, 2012, (3): 316-323.

JIN Li, ZHENG Hong-Chun, CHAI Tuan-Yao. Cloning and expression of PaOPT in Phytolacca americana[J]. , 2012, (3): 316-323.

参考文献

[1] Stacey M G, Koh S, Becker J, et al. AtOPT3, a member of the oligopeptide transporter family, is essential for embryo development in arabidopsis[J].The Plant Cell, 2002, 14: 2799–2811.
[2] Detmers F J M, Lanfermeijer F C, Poolman B. Peptides and ATP binding cassette peptide transporters[J].Research in Microbiology, 2001, 152(3/4): 245-258.
[3] Hosie A H, Poole P S. Bacterial ABC transporters of amino acids[J]. Research in Microbiology, 2001, 152: 259-270.
[4] Fehring R J, Lawrence D, Philpot C,et al. Use effectiveness of the creighton model ovulation method of natural family planning[J]. Journal of Obstetric, Gynecologic, Neonatal Nursing, 1994, 23(4):303-309.
[5] Waterworth W M, Bray C M. Enigma variations for peptides and their transporters in higher plants[J]. Annals of Botany, 2006, 98: 1-8.
[6] Williams L E, Miller A J. Transporters responsible for the uptake and partitioning of of nitrogenous solutes[J].Annual Review of Plant Physiology and Plant Molecular Biology, 2001, 52: 659-688.
[7] Stacey G, Koh S, Granger C, et al. Peptide transport in plants[J].Trends Plant Science, 2002, 7: 257-263.
[8] Tsay Y F, Chiu C C, Tsai C B, et al. Nitrate transporters and peptide transporters[J]. FEBS Letters, 2007, 581: 2290-2300.
[9] Pike S, Patel A, Stacey G, et al. Arabidopsis OPT6 is an oligopeptide transporter with exceptionally broad substrate specifi city[J]. Plant Cell Physiology, 2009, 50(11): 1923-1932.
[10] Lubkowitz M A, Hauser L, Hauser L, et al. An oligopeptide transport gene from Candida albicans [J]. Microbiology, 1997, 143: 387-396.
[11] Lubkowitz M A, Barnes D, Breslav M, et al. Schizosaccharomyces pombe isp4 encodes a transporter representing a novel family of oligopeptide transporters[J]. Molecular Microbiology, 1998, 28(4): 729-741.
[12] Hauser M, Donhardt A M, Barnes D, et al. Enkephalins are transported by a novel eukaryotic peptide uptake system[J].The Journal of Biological Chemistry, 2000, 275: 3037-3041.
[13] Stacey M G, Patel A, McClain W E, et al. The arabidopsis AtOPT3 protein functions in metal homeostasis and movement of iron to developing seeds[J]. Plant Physiology, 2008, 146: 589-601.
[14] Fei Y J, Romero M F, Krause M, et al. A novel H1-coupled oligopeptide transporter (OPT3) from caenorhabditis elegans with a predominant function as a H1 channel and an exclusive expression in neurons[J]. The Journal of Biological Chemistry, 2000, 275(18): 9563-9671.
[15] Stacey M G, Osawa H, Patel A, et al. Expression analyses of Arabidopsis oligopeptide transporters during seed germination, vegetative growth and reproduction[J]. Planta, 2006, 223: 291-305.
[16] 国家药典委员会.中华人民共和国药典[M]. 北京: 化学工业出版社, 2005.
[17] Nie F H. Cd hyper-accumulator Phytolacca acinosa Roxb and Cd-accumulative characteristics[J].Ecology and Environment,2006,15(2):303-306(in Chinese). 聂发辉.镉超富集植物商陆及其富集效应[J]. 生态环境,2006,15(2): 303-306.
[18] Xue S G,Chen Y X,Lin Q, et al. Phytolacca acinosa Roxb.(Phytolaceaceae):A new manganese hyper-aceumulator plant from Southern China[J].Acta Ecologica Sinica,2003,23(5):935-937(in Chinese). 薛生国,陈英旭, 林琦,等. 中国首次发现的锰超积累植物——商陆[J]. 生态学报,2003,23(5): 935-937.
[19] 吴双桃,朱慧. 商陆修复镉污染土壤初探[J]. 河北化工,2006,29(11): 58-60.
[20] Nie F H, Wu C B, Wu S T. Characteristics of phytolacca acinosa accumulating Cd[J]. Journal of ZheJiang Forestry College, 2006, 23(4):400-405(in Chinese). 聂发辉, 吴彩斌, 吴双桃. 商陆对镉的富集特征[J].浙江林学院学报,2006,23(4): 400-405.
[21] Dietrich D, Hammes U, Thor K, et al. AtPTR1, A plasma membrane peptide transporter expressed du ring seed germination and in v ascular tissue of Arabidopsis[J]. Plant J, 2004, 40(4): 488-499.
[22] Miranda M, Borisjuk L, Tewes A, et al. Peptide and amino acid transporters are differentially regulated during seed development and germination in faba bean[J]. Plant Physiol, 2003, 132: 1950-1960.
[23] 宋松泉,程红焱,姜孝成.种子生物学[M]. 北京:科学出版社,2008.

商陆寡肽转运蛋白基因PaOPT的 cDNA全序列克隆及表达分析

Cloning and expression of PaOPT in Phytolacca americana

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