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中国科学院大学学报 ›› 2002, Vol. 19 ›› Issue (4): 428-432.DOI: 10.7523/j.issn.2095-6134.2002.4.015

• 简报 • 上一篇    下一篇

低能N+离子注入谷氨酸产生菌诱变选育及其发酵的研究

甄卫军1, 2, 李茜3, 张石峰3, 曾宪贤3, 高林1, 李江4   

  1. 1. 中国科学院新疆化学所, 乌鲁木齐 830011;
    2 中国科学院研究生院, 北京 100039;
    3. 新疆大学离子束生物工程中心, 乌鲁木齐 830008;
    4. 新疆石河子味精厂, 石河子 832000
  • 收稿日期:2002-01-07 修回日期:2002-03-05 发布日期:2002-07-18
  • 作者简介:甄卫军, 男, 1969 年7 月生, 硕士生
  • 基金资助:

    新疆维吾尔自治区自然科学基金项目(20001);乌鲁木齐重大科技攻关项目(Y0010 )资助

Study of the Mutation Breeding and Fermentation on Glutamic Acid Yielding Bacterium with Low Energy N+Ion Beam Implantation

ZHEN Wei Jun1, 2, LI Qian3, ZHANG Shi Feng3, ZENG Xian Xian3, GAO Lin1, LI Jiang4   

  1. 1. Xinjiang Chemical Institute of the Chinese Academy of Sciences, Urumqi 830011;
    2. The Gradudate School of the Chinese Academy of Sciences, Beijing 100039;
    3. Center of Ion Beam Bioengineering, Xinjiang University, Urumqi 830008;
    4. Xinjiang Shihezi Monosodium Glutamate Plant, Shihezi 832000
  • Received:2002-01-07 Revised:2002-03-05 Published:2002-07-18

摘要:

采用低能N+离子注入技术,以D110 和D110B为出发菌进行了诱变选育高产谷氨酸菌株和发酵的试验研究。通过研究已经初筛到两株高产菌株D5221 和B3263,其多批次摇瓶平均产酸分别达到 8.4%、7.5 %,比出发菌分别提高 35.48%和 25 %.其一级种子生长曲线比原出发菌有明显变化,高产菌株发酵对数期平均提前 2~ 3h.从发酵曲线显示出高产菌株的代谢活力增强,倍增时间缩短,对缩短发酵周期有积极的促进作用

关键词: 离子注入, 谷氨酸, 诱变选育, 发酵

Abstract:

Low energy N+ion implantation technology is used in the mutat ion breeding and fermentation on g lutamic acid yielding start ing st rains of D110 and D110B.It is showed in this study that tw o high yielding glutamic acid st rains D5221 and B3263 are screened out and the average rocker bot tle fermentation GA(%)can achieve 8.4 %、7.5 %, they are respectively raised by 35.48 %、25 % compared to the original strains.There are clearly improved in the aspect on the fi rst class seed fermentation curve.And thei r log arithm phase has generally improved by 2 ~ 3 h.From the fermentation curve it is showed that thei r metabolic capacity are improved, the multiplicat ion cycle are shortened.So it w ill play a positive role in reducing the fermentation cycle.

Key words: ion implantation, glutamic acid, mutation breeding, fermentation

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