基于PBET和SHIME模型联用评估复合污染土壤中砷铅的生物可给性*

doi:10.7523/j.ucas.2026.001

摘要/Abstract

摘要： 经手-口摄入是土壤重金属人体暴露的重要途径之一。重金属的生物可给性是人体健康风险评估的关键参数。土壤砷（As）和铅（Pb）复合污染下,As和Pb的生物可给性及其相互影响研究较少。本研究采用3种不同pH的农田土壤（江西鹰潭JX、浙江嘉兴ZJ、内蒙古赤峰NM）,并人为添加As和Pb,老化1个月后形成不同浓度的As（50、100和200 mg·kg^-1）和Pb（200、400和800 mg·kg^-1）复合污染土壤,利用PBET和SHIME模型,探究了胃、小肠和结肠阶段As和Pb生物可给性的变化及相互影响。结果表明,As的生物可给性从胃到小肠阶段表现为JX土升高、ZJ土无显著变化、NM土降低,从小肠到结肠阶段整体呈升高趋势;Pb的生物可给性从胃到小肠到结肠阶段均显著降低（p<0.05）。胃和小肠阶段,As和Pb没有显著相互影响;在结肠阶段,随着Pb的添加及添加浓度的升高,As的生物可给性逐渐降低,在添加800 mg·kg^-1Pb时,3种土壤中As生物可给性降低16.8%~24.8%（p<0.001）,As（Ⅲ）浓度也逐渐降低。本研究结果明晰了As和Pb复合污染土壤中其生物可给性的变化及是否相互影响,对准确评估As和Pb复合污染土壤的人体健康风险具有重要的科学意义。

关键词: 砷, 铅, 复合污染土壤, 生物可给性, SHIME模型

Abstract: Hand-to-mouth ingestion is a significant pathway for human exposure to soil heavy metals. The bioaccessibility of heavy metals is a critical parameter for human health risk assessment. Research on the bioaccessibility of arsenic (As) and lead (Pb) and their potential interactions in co-contaminated soils is limited. This study utilized three farmland soils with different pH levels (Jiangxi Yingtan, JX; Zhejiang Jiaxing, ZJ; Inner Mongolia Chifeng, NM). As and Pb were artificially spiked into the soils, which were then aged for one month to create co-contaminated samples with varying concentrations of As (50, 100, and 200 mg·kg^-1) and Pb (200, 400, and 800 mg·kg^-1). The PBET and SHIME model were employed to investigate the changes and interactions in the bioaccessibility of As and Pb during the gastric, small intestinal, and colon phases. The results indicated that the bioaccessibility of As from the gastric to the small intestinal phase increased in JX soil, showed no significant change in ZJ soil, and decreased in NM soil. From the small intestinal to the colon phase, As bioaccessibility exhibited an overall increasing trend. In contrast, the bioaccessibility of Pb decreased significantly (p < 0.05) throughout the progression from the gastric to the small intestinal and then to the colon phase. No significant interaction between As and Pb was observed during the gastric and small intestinal phases. However, in the colon phase, the bioaccessibility of As gradually decreased with the addition and increasing concentration of Pb. At the highest Pb addition level (800 mg·kg^-1), the bioaccessibility of As in the three soils was reduced by 16.8% to 24.8% (p < 0.001), accompanied by a decrease in the concentration of As(Ⅲ). These findings clarify the variations in bioaccessibility and the interaction between As and Pb in co-contaminated soils, providing important scientific insights for the accurate assessment of human health risks associated with As and Pb co-contamination.

Key words: arsenic, lead, co-contaminated soils, bioaccessibility, SHIME model

中图分类号:

闫士博, 徐则林, 田稳, 常旭卉, 杨茂林, 蔡晓琳, 尹乃毅, 崔岩山. 基于PBET和SHIME模型联用评估复合污染土壤中砷铅的生物可给性^*[J]. 中国科学院大学学报, DOI: 10.7523/j.ucas.2026.001.

YAN Shibo, XU Zelin, TIAN Wen, CHANG Xuhui, YANG Maolin, CAI Xiaolin, YIN Naiyi, CUI Yanshan. Assessment of arsenic and lead bioaccessibility in co-contaminated soil using a coupled PBET-SHIME model[J]. Journal of University of Chinese Academy of Sciences, DOI: 10.7523/j.ucas.2026.001.

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基于PBET和SHIME模型联用评估复合污染土壤中砷铅的生物可给性^*

Assessment of arsenic and lead bioaccessibility in co-contaminated soil using a coupled PBET-SHIME model

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