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中国科学院大学学报 ›› 2014, Vol. 31 ›› Issue (1): 46-53.DOI: 10.7523/j.issn.2095-6134.2014.01.008

• 化学与生命科学 • 上一篇    下一篇

一氧化氮对新疆紫草愈伤组织中紫草素及其衍生物合成的影响

郝鹤1, 叶和春2, 王红1   

  1. 1. 中国科学院大学生命科学学院, 北京 100049;
    2. 中国科学院植物研究所, 北京 100093
  • 收稿日期:2013-04-01 修回日期:2013-04-19 发布日期:2014-01-15
  • 通讯作者: 王红,E-mail:hwang@ucas.ac.cn
  • 基金资助:

    国家自然科学基金(61173098)和中国科学院“十二五”基础前沿专项(KSCX2-EW-J-29)资助

Effects of nitric oxide on the biosynthesis of shikonin and its derivatives in callus of Arnebia euchroma

HAO He1, YE Hechun2, WANG Hong1   

  1. 1 College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China;
    2 Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
  • Received:2013-04-01 Revised:2013-04-19 Published:2014-01-15

摘要:

一氧化氮(NO)是一种在植物次生代谢中起着重要调控作用的信号分子. 我们以硝普钠(SNP)作为 NO 供体处理新疆紫草愈伤组织. 实验表明,SNP 处理的新疆紫草红色愈伤组织中AePGT,AeGPPS,AeC4HAe4CL的表达量比未处理组明显提高. 对萘醌类代谢物谱的分析显示,SNP 处理也引起新疆紫草愈伤组织中紫草素及其衍生物含量的增加. 上述结果表明,SNP 处理产生的 NO,可能通过上调紫草素及其衍生物合成相关的基因表达,促进紫草素及其衍生物的合成.

关键词: 新疆紫草, 一氧化氮, 紫草素及其衍生物, 硝普钠, 基因表达

Abstract:

Nitric oxide (NO) is an important signal molecule involved in the regulation of plant secondary metabolism. In this study, SNP (sodium nitroprusside) was utilized as the donor of nitric oxide to treat Arnebia euchroma callus. We find that the expression levels of the genes involved in the biosynthesis of shikonin and its derivatives, such as AePGT, AeGPPS, AeC4H, and Ae4CL, increase significantly in SNP-treated Red Strain callus tissues. Meanwhile, SNP treatment also increases the contents of some shikonin derivatives. The above-mentioned results indicate that exogenous NO increases the biosynthesis of shikonin and its derivatives by up-regulating the expression of the genes.

Key words: Arnebia euchroma, nitric oxide, shikonin and its derivatives, sodium nitroprusside, gene expression

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