红豆杉科及三尖杉科的分子系统发育——兼论竹柏属的系统位置

中国科学院大学学报 ›› 2000, Vol. 38 ›› Issue (3): 201-210.

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红豆杉科及三尖杉科的分子系统发育——兼论竹柏属的系统位置

汪小全,舒艳群

收稿日期:1900-01-01 修回日期:1900-01-01 发布日期:2000-05-10
通讯作者: 汪小全

Chloroplast matK gene phylogeny of Taxaceae and Cephalotaxaceae, with additional reference to the systematic position of Nageia

WANG Xiao-Quan, SHU Yan-Qun

Received:1900-01-01 Revised:1900-01-01 Published:2000-05-10
Contact: WANG Xiao-Quan

摘要/Abstract

摘要：

测定了红豆杉科、三尖杉科、罗汉松科及松科共9属13种植物的matK基因序列，发现3′端的单碱基插入造成mat k基因在这些类群间有较大的长度变异(1488∽1548bp)，其它插入或缺失的长度为3、6或9bp(白豆杉中有一个长为27bp的缺失)，且绝大多数插入或缺失事件具有系统发育信息。matK基因中，密码子第1、2及3位的变异率较为相近，平均同义替代率约为平均非同义替代率的2倍。以松科2属3种植物为外类群，运用PAUP软件进行的系统发育分析仅得到一棵最简约树(步长为895，CI=0．850，RI=0．876)，该简约树的各分支均得到bootstrap分析的极强支持。结果表明：红豆杉科与三尖杉科均为单系群，二者互为姐妹群；白豆杉属与穗花杉属为红豆杉科中两个很自然的属，白豆杉属为红豆杉属的姐妹群，穗花杉属为榧树属的姐妹群。竹柏属与罗汉松属聚为一支，二者间的亲缘关系得到boot-strap分析的100％支持，从分支分类学及遗传距离角度，作者不赞同将竹柏属提升为科。

关键词: 红豆杉科, 三尖杉科, 竹柏属, matK 基因, 分子系统学

Abstract:

Reported in the present paper is a robust chloroplast matK gene phylogeny of Taxaceae,

Cephalotaxaceae and Podocarpaceae represented by 10 species of seven genera, with three species of

the Pinaceae as outgroups. The matk length of the 13 species ranges from 1488 bp to 1548 bp,

which results from indels, in particular, 1-bp(base pair) insertion near the 3’ end of the gene in

some groups. A 27 bp deletion was found at the nucleotide position 213 from the 5’ end of the matk

gene of Pseudotaxus chienii. The aligned sequences used in PAUP and MEGA analyses were 1568

bp and 1494 bp respectively. In the matK gene, the rates of variation at the first, second and third

codon positions are similar although the mean frequency of synonymous substitution is approximately

twice as high as that of nonsynonymous substitution. Branch-and-Bound search found only one most

parsimonious tree (tree length = 895, CI = 0.850, RI = 0. 876), in which all clades were strongly

supported by bootstrap test. According to the tree, Taxaceae and Cephalotaxaceae are monophyletic

groups, and the sister group relationship between the two families was confirmed. Taxus is closely

related to Pseudotaxus while Torreya is the sister group of Amentotaxus. In addition, the close rela-

tionship between Nageia and Podocarpus was resolved. The present study supports the generic sta-

tus of Pseudotaxus and Amentotaxus in point of cladistic analysis and genetic distance, but contra-

dicts the establishment of the family Nageiaceae.

Key words: Taxaceae, Cephalotaxaceae, Nageia；matK, Molecular systematics

汪小全,舒艳群. 红豆杉科及三尖杉科的分子系统发育——兼论竹柏属的系统位置[J]. 中国科学院大学学报, 2000, 38(3): 201-210.

WANG Xiao-Quan, SHU Yan-Qun. Chloroplast matK gene phylogeny of Taxaceae and Cephalotaxaceae, with additional reference to the systematic position of Nageia[J]. , 2000, 38(3): 201-210.

红豆杉科及三尖杉科的分子系统发育——兼论竹柏属的系统位置

Chloroplast matK gene phylogeny of Taxaceae and Cephalotaxaceae, with additional reference to the systematic position of Nageia

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