山羊草属异源多倍体物种核rDNA ITS区的进化

中国科学院大学学报 ›› 2000, Vol. 38 ›› Issue (3): 211-217.

山羊草属异源多倍体物种核rDNA ITS区的进化

王超,王建波,施苏华,钟扬

收稿日期:1900-01-01 修回日期:1900-01-01 发布日期:2000-05-10
通讯作者: 王超

Evolution of Internal Transcribed Spacer Region of Nuclear Ribosomal DNA in Allopolyploids of Aegilops

WANG Chao, WANG Jian-Bo, SHI Su-Hua, ZHONG Yang

Received:1900-01-01 Revised:1900-01-01 Published:2000-05-10
Contact: WANG Chao

摘要/Abstract

摘要：

本文测定了山羊草属Aegilops 3个组中异源多倍体物种的核rDNA ITS区序列，并用邻接法进行了聚类分析。结果表明，多倍体物种的ITS区序列长度为559∽606bp，其中ITS1、ITS2分别有变异位点51、42个，且存在多态位点。多倍体种均与各自的某一祖先种构成稳定分支，说明在杂交-多倍化后，这些多倍体的ITS区在同步进化的作用下已向着其某一祖先种的ITS区进化。对于sect．Vertebrata的异源多倍体物种来说，其ITS区主要向其祖先种Ae．umbellulata(UU)的ITS区进化，这与山羊草属的细胞遗传学研究结果基本一致。在sect．Cylindropyrum和sect．Polyeides中，Ae．cylindrica(CCDD)朝着Ae．caudata (CC)进化；Ae．ventricosa(DDMvMv)朝着Ae．comosa(MM)进化；Ae．vavilovii(DDMMSS)朝着Ae．crassa (DDMM)进化。

关键词: 山羊草属, 异源多倍体, ITS区序列, 进化

Abstract:

Hybridization with subsequent polyploidy is a prominent process in evolution of higher

plants, but few data address the evolution of homeologous sequences after polyploidy. The internal

transcribed spacer (ITS) of nuclear ribosomal DNA (nrDNA) from eleven allopolyploid species in

Aegilops was investigated by PCR amplification and direct sequencing. The sequences obtained were

used to study the evolution of ITS region in allopolyploid species. The length of ITS region varied

from 599 to 606 bp and the number of variable sites was 93, i.e. 51 and 42 for ITS1 and ITS2 re-

spectively. Some polymorphic sites were observed in polyploid species, and this indicated that the

ancestral sequences had not been homogenized completely by concerted evolution. Distance matrix

analysis of diploid and polyploid species by neighbor-joining method, using Triticum monococcum as

outgroup, resulted in well-resolved neighbor-joining tree indicating that the ITS regions of UUMM

and UUSS genome ( sect. Vertebrata) were homogenizing toward those of UU ancestal genome. This

result is in agreement with the results of ctyogenetics of Aegilops. On the other hand, the neighbor-

joining tree including the D-genome group species (sect. Cylindropyrum and sect. Polyeides ) com-

prised three clades (CC-DDCC, UU-DDMM-DDMMSS-DDMMUU and MM-DDMvMv), which sug-

gested that concerted evolution was homogenizing the ITS region of the polyploid derivatives to either

of their ancestors.

Key words: Aegilops, Allopolyploid, ITS sequence, Evolution

王超,王建波,施苏华,钟扬. 山羊草属异源多倍体物种核rDNA ITS区的进化[J]. 中国科学院大学学报, 2000, 38(3): 211-217.

WANG Chao, WANG Jian-Bo, SHI Su-Hua, ZHONG Yang. Evolution of Internal Transcribed Spacer Region of Nuclear Ribosomal DNA in Allopolyploids of Aegilops[J]. , 2000, 38(3): 211-217.

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