关键词: 低能离子, AL细胞, 生物效应, 甘油, 保护

Abstract:

The discovery of bio-ef fects induced by ion implantation has opened a novel branch in the field of radiation biology ———the low energy heavy ion biology.The experimental samples in the field have, however, been within the limits of plants and microbes because of the sample's vacuum-resistant ability.The mutation studying of mammalian cells with ion implantation hasn't been reported in the past years.The main reason for that is that it is difficult to keep mammalian cells alive in vacuum during ion implantation.In the present study, the tolerance of mammalian cells in vacuum was enhanced efficiently by pre-treatment with glycerin at low concent rat ion and proper treatment time, which provides a new way for the study on the biological effects of ion beam implantation in the mammalian cells.

Key words: low energy ions, AL cell, bio-effects, glycerine, protection