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›› 2013, Vol. 30 ›› Issue (1): 40-46.DOI: 10.7523/j.issn.1002-1175.2013.01.007

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Detection of selected pathogenic bacteria during oxidation ditch wastewater treatment

LIN Yu-Xin, ZHANG Zhen-Nan, XU Ya-Hui, LI Juan, LIU Xin-Chun   

  1. College of Resources and Environment, Graduate University, Chinese Academy of Sciences, Beijing 100049, China
  • Received:2012-01-13 Revised:2012-03-27 Online:2013-01-15

Abstract:

Indicator bacterium E. coli and pathogenic bacteria Salmonella and Legionella were detected during the wastewater treatment of oxidation ditch, using SYBR I Real-time quantitative PCR. The detected amount of E.coli was 108 copies/mL and decreased by 2 orders of magnitude eventually. In the initial stages, the amount of Salmonella ranged from 102 to 103 copies/mL but was eliminated or reduced to undetectable level eventually. The amount of Legionella was detected to be 10-102 copies/mL in the sample of activated sludge. However, the amount of Legionella in the effluent samples was detectable and similar to those in the influent samples, which ranged from 104 to 105 copies/mL.

Key words: oxidation ditch, pathogenic bacteria, real-time quantitative PCR

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