Purification and characterization of β-glucosidase from Aspergillus niger

doi:10.7523/j.issn.2095-6134.2009.2.018

Abstract

Abstract:

A high activity glucosidase, which can hydrolyze glycosides, was isolated from Aspergillus niger S strain. The enzyme was purified to one spot after ammonium sulfate precipitation, ion exchange & size exclusion chromatography and its molecular weight was about 165ku, which was quite differ from other reported glucosidase. The optimum temperature of this enzyme was 50℃ & the optimum pH was pH 5~10. Based on the HPLC result, this enzyme can transform glycone to aglycone of isoflavones & resveratrol with high specific activity.

Key words: soybean isoflavones, resveratrol, glucosidase, purification, characterization

CLC Number:

WANG Yun, ZENG Wo-Tan. Purification and characterization of β-glucosidase from Aspergillus niger[J]. , 2009, 26(2): 274-279.

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