Inhibitory effects of strawberry extracts on animal fatty acid synthase

doi:10.7523/j.issn.2095-6134.2010.6.006

Abstract

Abstract:

Fatty acid synthase (FAS) has been considered as a potential new therapeutic target for obesity. In the present study we show that the components of both strawberry ( Fragaria ananassa Duch) fruit dreg and strawberry leaves that extracted with 50% ethanol and partitioned by ethyl acetate are potent FAS inhibitors. The ethyl acetate fractions of strawberry fruit dregs (SF-AE) show noncompetitive inhibition with the substrate Mal-CoA, while the ethyl acetate fractions of strawberry leaves (SL-AE) show competitive inhibition with Ac-CoA. Based on the HPLC-MS analysis, we conclude that ellagic acid and quercetin are the efficient components. SF-AE and SL-AE could reduce lipids accumulation in 3T3-L1 preadipocyte with dose-dependence. These results may be of great value for application of strawberry extracts to obesity prevention and cure.

Key words: fatty acid synthase, strawberry, inhibitor, kinetic assay, anti-obesity

CLC Number:

R285.5

LIU Xiao-Xin, TIAN Wei-Xi, MA Xiao-Feng. Inhibitory effects of strawberry extracts on animal fatty acid synthase[J]. , 2010, 27(6): 768-777.

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