Identification and biological property study of an enterohemorrhagic E.coli O157 phage isolated from sewage treatment plant aeration tank

doi:10.7523/j.issn.2095-6134.2015.04.013

Abstract

Abstract: The purpose of this work was to isolate the lytic phages of Escherichia coli O157 from an aeration tank sample and to investigate the biological characteristics of the isolated phage. The titer of purified bactiriophage was 8.7×10¹¹pfu/mL. The electron microscope showed the phage had a hexahedron cubic-symmetric head with 70 nm in diameter and a short tail (about 10 nm in length and about 10 nm in width). The restriction enzyme fragments suggested that it was dsDNA. The optimal MOI (multiplicity of infection) value was 0.01. Phage PO157-Z showed wide range of acid-alkali tolerance. In addition, the curves of bacteria lysed by phage showed that PO157-Z had a high lytic activity on E.coli O157. Thus, PO157-Z is a strain of lytic phage against enterohemorrhagic E.coli O157 and belongs to tailed families Podoviridae with dsDNA. These characteristics of phage PO157-Z would be useful in various applications as a novel biocontrol agent against E.coli O157.

Key words: bacteriophage, Escherichia coli O157, wastewater treatment system, biocontrol

CLC Number:

X703

YI Xin, LIU Xinchun, HUANG Jing, LIU Honghui, LI Juan. Identification and biological property study of an enterohemorrhagic E.coli O157 phage isolated from sewage treatment plant aeration tank[J]. , 2015, 32(4): 512-519.

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