Mutagenesis, screening, and medium optimization of high[(4'-OH)MeLeu]4-CsA producing strain-Nonomuraea dietziae

doi:10.7523/j.issn.2095-6134.2017.05.005

Abstract

Abstract: An ultraviolet-lithium chloride mutagenesis strategy was carried out on the[(4'-OH)MeLeu]⁴-CsA producing strain, Nonomuraea dietziae.The production of mutant strain reached 208mg/L and increased by 26.8%. Then the fermentation medium was optimized by single-factor and response surface methods. The optimal medium was determined as follows (materials/concentrations in g/L):glycerol/26.5, dextrin/11.6, peptone/9.7, yeast extract powder/3.5, soybean meal/15.0, soybean oil/2.0, MgSO₄·7H₂O/0.40, MnSO₄·H₂O/0.010, and (NH₄)₂MoO₄/0.010. The final production reached 324mg/L and increased by 97.6% compared with the initial value.

Key words: Nonomuraea dietziae, [(4'-OH)MeLeu]⁴-CsA, ultraviolet lithium chloride mutagenesis, response surface optimization

CLC Number:

Q939.9

JIN Lina, WEN Jianping. Mutagenesis, screening, and medium optimization of high[(4'-OH)MeLeu]⁴-CsA producing strain-Nonomuraea dietziae[J]. , 2017, 34(5): 558-566.

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Mutagenesis, screening, and medium optimization of high[(4'-OH)MeLeu]⁴-CsA producing strain-Nonomuraea dietziae

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