Effects of nitrogen and phosphorus application on soil microbial biomass carbon and nitrogen contents on an alpine grassland on Tibetan Plateau

doi:10.7523/j.issn.2095-6134.2018.03.017

Abstract

Abstract: Soil microbes play an important role in maintaining and improving plant productivity as well as soil quality. Based on a two-year field experiment of fertilizer application to an alpine steppe on the Tibetan Plateau, effects of nitrogen and phosphorus addition on soil microbial biomass carbon (MBC) and nitrogen (MBN) contents in the upper 30cm soil were studied. The results are given as follows. 1) Nitrogen and phosphate addition influenced soil MBC content mainly in the 0-10cm soil layer, and the content was 1.67 times and 1.28 times the contents in the 10-20cm and 20-30cm soil layers, respectively. 2) Sole nitrogen addition significantly decreased soil MBC and MBN contents, whereas nitrogen application in addition to phosphorus fertilization increased soil MBC and MBN contents. 3) Phosphorus fertilization alone had no significant effect on soil MBC and MBN contents. Combined with nitrogen fertilizer at the N₂ level of 15g N/m², phosphorus application at the levels of 15 and 30g P₂O₅/m² (i.e., P₂ and P₃ levels) significantly increased MBC content in the 0-10cm soil layer and MBN content in the 10-20cm soil layer, as compared with control treatment. Furthermore, MBN content in the 10-20cm soil layer was also significantly higher in the N₂P₁ (15g N/m² and 7.5g P₂O₅/m²) treatment than CK treatment.

Key words: alpine steppe, phosphorus addition, microbial biomass carbon, microbial biomass nitrogen

CLC Number:

S158
S812.2

ZHAO Guoqiang, WANG Shuping, CUI Xiaoyong, DONG Junfu, CHANG Xuhui, WANG Shiping. Effects of nitrogen and phosphorus application on soil microbial biomass carbon and nitrogen contents on an alpine grassland on Tibetan Plateau[J]. , 2018, 35(3): 417-424.

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