Species identification of palm leaf manuscript based on Py-GC/MS

doi:10.7523/j.ucas.2024.078

Abstract

Abstract: The materials for making palm leaf manuscripts mainly come from the leaves of two palms, Corypha umbraculifera and Borassus flabellifer. Applying a micro-destructive and rapid method to determine the plant species origin is conducive to in-depth cognition of the utilization of the palm leaf manuscripts and provides a reference for its future conservation. In this study, a batch of standard samples were produced through simulation experiments to search the criteria to distinguish the two palm species and validate them on the artifacts of the palm leaf manuscripts of known plant species. Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) was utilized in combination with multivariate statistical analysis to differentiate the C. umbraculifera and B. flabellifer. The results revealed the presence of several triterpenoids in the samples, among which cycloeucalenol derivatives were found only in C. umbraculifera; two lupane-type triterpenoids and one cyclolaudenol and hopane-type triterpenoids were found only in B. flabellifer. The lupane-type triterpene and hopane-type compounds are stable in B. flabellifer. The relative contents of 10 lignin monomers were selected for principal component analysis (PCA), and the results showed a good differentiation between the C. umbraculifera group and the B. flabellifer group. The combination of the characteristic compounds and the PCA method successfully identified different types of plant sources of modern palm leaf manuscripts, which provides a new idea for the rapid and trace identification study of the species and genera of ancient palm leaf manuscripts.

Key words: palm leaf manuscript, Py-GC/MS, species identification

CLC Number:

CHEN Qingle, YANG Yimin, Han Bin, JIANG Hongen. Species identification of palm leaf manuscript based on Py-GC/MS[J]. Journal of University of Chinese Academy of Sciences, DOI: 10.7523/j.ucas.2024.078.

References

[1] 李学竹. 中国梵文贝叶经概况[J]. 中国藏学, 2010(S1): 53-62.
[2] 张美芳, 宋欣, 吕晓芳, 等. 西藏贝叶经主要病害的诊断及修复关键技术的分析[J]. 中国藏学, 2022(4): 165-178.
[3] Chu S M, Lin L Y, Tian X L.Evaluation of the deterioration state of historical palm leaf manuscripts from Burma[J]. Forests, 2023, 14(9): 1775. DOI: 10.3390/f14091775.
[4] Lian X D, Yu C, Han W W, et al.Revealing the mechanism of ink flaking from surfaces of palm leaves (Corypha umbraculifera)[J]. Langmuir, 2024, 40(12): 6375-6383. DOI: 10.1021/acs.langmuir.3c03946.
[5] 杜宝. 大业杂记1卷[M]. 金山钱氏据借月山房量钞残版重编曾刻指海本. 1841(道光二十一年).
[6] 张仲裁. 酉阳杂俎[M]. 北京: 中华书局, 2017.
[7] Sah A.Palm leaf manuscripts of the world: Material, technology and conservation[J]. Studies in Conservation, 2002, 47(Supplement-1): 15-24. DOI: 10.1179/sic.2002.47.supplement-1.15.
[8] Sharma D, Singh M, Krist G, et al. Structural characterisation of 18th century Indian palm leaf manuscripts of India[J/OL]. International Journal of Conservation Science, 2018, 9(2): 257-264. (2020-07-15)[2024-8-10]. https://ijcs.ro/public/IJCS-18-24_Sharma.pdf.
[9] Freeman R. Turning over old leaves: Palm leaves used in South Asian manuscripts[J/OL]. The Book and Paper Group Annual, 2005, 24: 99-102. (2021-11-10)[2024-08-10]. https://cool.culturalheritage.org/coolaic/sg/bpg/annual/v24/bpga24-17.pdf#:~:text=leaf%20color%20and%20degree%20of%20%EF%AC%82exibility.
[10] Suryawanshi D G, Sinha P M, Agrawal O P.Basic studies on the properties of palm leaf[J]. Restaurator. International Journal for the Preservation of Library and Archival Material, 1994, 15(2): 65-78. DOI: 10.1515/rest.1994.15.2.65.
[11] Degano I, Modugno F, Bonaduce I, et al.Recent advances in analytical pyrolysis to investigate organic materials in heritage science[J]. Angewandte Chemie (International Ed), 2018, 57(25): 7313-7323. DOI: 10.1002/anie.201713404.
[12] Avataneo C, Sablier M.New criteria for the characterization of traditional East Asian papers[J]. Environmental Science and Pollution Research, 2017, 24(3): 2166-2181. DOI: 10.1007/s11356-016-6545-0.
[13] 姚娜. 基于热裂解气相色谱质谱法的明清时期纸质文物科学研究[D]. 北京: 北京科技大学, 2022. DOI: 10.26945/d.cnki.gbjku.2022.000150.
[14] Han B, Vial J, Sakamoto S, et al.Identification of traditional East Asian handmade papers through the multivariate data analysis of pyrolysis-GC/MS data[J]. Analyst, 2019, 144(4): 1230-1244. DOI: 10.1039/c8an01898g.
[15] Alahakoon C N K. Indigenous conservation practices for palm-leaf manuscripts in India[M]//Gopalkrishnan S. Samiksika series I: indigenous methods and manuscript preservation. New Delhi: D.K. Printworld Pvt Ltd. 2006: 61-73.
[16] Kumar D U, Sreekumar G, Athvankar U. Traditional writing system in southern India—palm leaf manuscripts[J/OL]. Design Thoughts (IDC, IIT Bombay), 2009, 2: 2-7. (2009-05-11)[2024-08-10]. https://www.idc.iitb.ac.in/resources/dt-july-2009/Palm.pdf#:~:text=Traditional%20writing%20system%20in%20Southern%20India.
[17] Ralph J, Hatfield R D.Pyrolysis-GC-MS characterization of forage materials[J]. Journal of Agricultural and Food Chemistry, 1991, 39(8): 1426-1437. DOI: 10.1021/jf00008a014.
[18] Saiz-Jimenez C, De Leeuw J W. Lignin pyrolysis products: Their structures and their significance as biomarkers[J]. Organic Geochemistry, 1986, 10(4/5/6): 869-876. DOI: 10.1016/S0146-6380(86)80024-9.
[19] Shiojima K, Arai Y, Masuda K, et al.Mass spectra of pentacyclic triterpenoids[J]. Chemical and Pharmaceutical Bulletin, 1992, 40(7): 1683-1690. DOI: 10.1248/cpb.40.1683.
[20] Jacob J, Disnar J R, Boussafir M, et al.Pentacyclic triterpene methyl ethers in recent lacustrine sediments (Lagoa do Caçó, Brazil)[J]. Organic Geochemistry, 2005, 36(3): 449-461. DOI: 10.1016/j.orggeochem.2004.09.005.
[21] Antonova G F, Varaksina T N, Zheleznichenko T V, et al.Changes in lignin structure during earlywood and latewood formation in Scots pine stems[J]. Wood Science and Technology, 2019, 53(4): 927-952. DOI: 10.1007/s00226-019-01108-w.
[22] Guerin C, Serret J, Montúfar R, et al.Palm seed and fruit lipid composition: Phylogenetic and ecological perspectives[J]. Annals of Botany, 2020, 125(1): 157-172. DOI: 10.1093/aob/mcz175.
[23] Funamoto Y, Yamauchi N, Shigenaga T, et al.Effects of heat treatment on chlorophyll degrading enzymes in stored broccoli (Brassica oleracea L.)[J]. Postharvest Biology and Technology, 2002, 24(2): 163-170. DOI: 10.1016/S0925-5214(01)00135-1.